Departamento de Bioquímica Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano # 1, Tlalpan, México DF014080, México.
J Bioenerg Biomembr. 2010 Oct;42(5):381-6. doi: 10.1007/s10863-010-9300-0. Epub 2010 Aug 20.
Ca²+ loading in mitochondria promotes the opening of a non-selective transmembrane pathway. Permeability transition is also associated with the interaction of cyclophilin D at the internal surface of the non-specific transmembrane pore. This interaction is circumvented by cyclosporin A and ADP. Our results show that, in the absence of ADP, liver mitochondria were unable to retain Ca²+, they underwent a fast and large amplitude swelling, as well as a rapid collapse of the transmembrane potential. In contrast, in the absence of ADP, kidney mitochondria retained Ca²+, swelling did not occur, and the collapse of the membrane potential was delayed. Ca²+ efflux was reversed by the addition of ADP and cyclosporin A. Our findings indicate that the differences between liver and kidney mitochondria are due to the low association of cyclophilin D to the ADP/ATP carrier found in kidney mitochondria as compared to liver mitochondria.
钙离子在线粒体中的加载促进了非选择性跨膜途径的开放。通透性转换也与亲环素 D 在非特异性跨膜孔内部表面的相互作用有关。环孢素 A 和 ADP 可以避免这种相互作用。我们的结果表明，在没有 ADP 的情况下，肝线粒体无法保留 Ca²+，它们会经历快速且幅度较大的肿胀，以及跨膜电位的快速下降。相比之下，在没有 ADP 的情况下，肾线粒体保留了 Ca²+，肿胀没有发生，膜电位的下降也被延迟。添加 ADP 和环孢素 A 可以逆转 Ca²+的外流。我们的发现表明，肝线粒体和肾线粒体之间的差异是由于肾线粒体中与 ADP/ATP 载体结合的亲环素 D 较少，而肝线粒体中则较多。
