Department of Biomaterials, Institute for Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt, Germany.
J Biomed Mater Res A. 2010 Dec 1;95(3):691-700. doi: 10.1002/jbm.a.32888.
Recently described organic-inorganic nanocomposite coatings of the chemical composition: (PLL/PGA)(10)CaP(PLL/PGA)(5)CaP (coating A) and (PLL/PGA)(10)CaP(PLL/PGA)(5)CaP(PLL/PGA)(5) (coating B), applied to chemically etched titanium plates, have been tested by extensive cell culture tests and in vivo biological experiments, with uncoated titanium plates serving as controls. Before testing, coated samples were stored for extended periods of time (from 2 weeks to 8 months) under dry, sterile conditions. Cells of the cell-lines MC3T3-E1 and/or SAOS-2 were used for the following cell culture tests: initial adhesion (4 h) and proliferation (up to 21 days), cell activity (XTT test), morphology, synthesis of collagen type I and alkaline phosphatase activity (all incubation up to 21 days). In addition, coating B was tested against uncoated control in a validated in vivo pull-out model in rabbit tibia. The results of both in vitro and in vivo experiments show excellent biological properties of chemically etched titanium which are even surpassed by surfaces covered with coating B. Thus, after 8 weeks of healing the implants coated with B were significantly better attached to the cortical bone of rabbit thibiae than uncoated titanium controls with more than twice the force needed to detach coated implants. However, coating A (top crystal layer) had an adverse effect on both cell proliferation and activity, which is explained by morphological observations, showing inhibited spreading of the cells on its rough surfaces. The results also show the remarkable stability of the coatings when shelved under dry and sterile conditions.
最近描述的化学组成的有机-无机纳米复合涂层:( PLL / PGA )( 10 ) CaP[( PLL / PGA )( 5 ) CaP ]( 4 )(涂层 A )和( PLL / PGA )( 10 ) CaP[( PLL / PGA )( 5 ) CaP ]( 4 )( PLL / PGA )( 5 )(涂层 B ),应用于化学蚀刻的钛板,已被广泛的细胞培养试验和体内生物实验测试,未涂层的钛板作为对照。在测试之前,涂层样品在干燥无菌条件下储存了很长时间(从 2 周到 8 个月)。细胞的细胞系 MC3T3 - E1 和/或 SAOS-2 用于以下细胞培养试验:初始粘附( 4 小时)和增殖(长达 21 天),细胞活性( XTT 试验),形态,胶原 I 合成和碱性磷酸酶活性(所有孵育长达 21 天)。此外,涂层 B 在兔胫骨中经过验证的体内拔出模型中进行了未经处理的对照测试。体外和体内实验的结果均显示出化学蚀刻钛的优异生物学特性,甚至超过了用涂层 B 覆盖的表面。因此,经过 8 周的愈合,用 B 涂层的植入物与未经处理的钛对照物相比,与皮质骨的附着明显更好,需要分离涂层植入物的力超过两倍。然而,涂层 A (顶层晶体层)对细胞增殖和活性有不利影响,这可以从形态学观察结果得到解释,表明细胞在其粗糙表面上的扩散受到抑制。结果还表明,涂层在干燥无菌条件下搁置时具有显著的稳定性。