[双反义淀粉分支酶基因转基因水稻亚慢性毒性试验]

Li Min, Piao Jianhua, Yang Xiaoguang

Key Laboratory of Trace Element Nutrition MOH, Institute of Nutrition and Food Safety, Chinese CDC, Beijing 100050, China.

Wei Sheng Yan Jiu. 2010 Jul;39(4):436-9, 443.

OBJECTIVE

To observe the sub-chronic toxic effects of the genetically modified rice with double antisense SBE gene.

METHODS

Based on gender and weight, weanling Wistar rats were randomly sorted into five groups: non-genetically modified rice group (group A), genetically modified rice group (group B), half genetically modified rice group (group C), quarter genetically modified rice group (group D) and AIN-93G normal diet group (group E). Indicators were the followings: body weight, food consumption, blood routine, blood biochemical test, organ weight, bone density and pathological examination of organs.

RESULTS

At the middle of the experiment, the percentage of monocyte of female group B was less than that of group E (P < 0.05). AST activity of female group B was higher than that of group E (P < 0.05). ALT activity of female group C was higher than that of groups A and E (P < 0.05). At the end of the experiment, AST and ALT activities of female group B were higher than those of group E (P < 0.05). Hematocrit (HCT) and blood urea nitrogen (BUN) level of male group B were less than those of group A (P < 0.05), and monocyte was higher than that of group E (P < 0.05). The brain index of female group B was higher than group A (P < 0.05) and the kidney index of group E were higher than those of groups B, C and D (P < 0.05). There were no significant difference of blood lipids, calcium and bone mineral density among all groups (P > 0.05), and no notable abnormity in the pathological examination of main organs (P > 0.05).

CONCLUSION

There were no enough evidence to confirm the sub-chronic toxicity of genetically modified rice on rats.

目的

观察转双反义淀粉分支酶基因（SBE）水稻的亚慢性毒性作用。

方法

将断乳Wistar大鼠按性别、体重随机分为五组：非转基因水稻组（A组）、转基因水稻组（B组）、半量转基因水稻组（C组）、四分之一量转基因水稻组（D组）和AIN-93G正常饲料组（E组）。观察指标包括体重、进食量、血常规、血液生化检测、脏器重量、骨密度及脏器病理检查。

结果

实验中期，B组雌性大鼠单核细胞百分比低于E组（P<0.05）；B组雌性大鼠谷草转氨酶（AST）活性高于E组（P<0.05）；C组雌性大鼠谷丙转氨酶（ALT）活性高于A组和E组（P<0.05）。实验末期，B组雌性大鼠AST和ALT活性高于E组（P<0.05）；B组雄性大鼠血细胞比容（HCT）和血尿素氮（BUN）水平低于A组（P<0.05），单核细胞高于E组（P<0.05）；B组雌性大鼠脑指数高于A组（P<0.05），E组大鼠肾指数高于B、C、D组（P<0.05）。各实验组血脂、血钙及骨密度比较差异均无统计学意义（P>0.05），主要脏器病理检查未见明显异常（P>0.05）。