Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Diagn Microbiol Infect Dis. 2010 Sep;68(1):50-4. doi: 10.1016/j.diagmicrobio.2010.04.004.
Detection of specific IgM antibodies by ELISA forms the mainstay for diagnosis of dengue infection. However, IgM antibodies develop after 4 to 5 days of infection. The methods for early diagnosis include virus isolation and reverse transcriptase polymerase chain reaction (RT-PCR) which need a sophisticated laboratory. Another alternative that has recently come up is NS1 antigen detection. The present study compared IgM antibody detection with NS1 antigen for the diagnosis of acute dengue in 87 samples. NS1 antigen could be detected with good sensitivity (71-100%) till day 3 of fever, whereas IgM had a sensitivity of 0% to 50% at this time. On day 4 of illness, both the tests had comparative sensitivity. Beyond day 4, IgM antibody detection was superior to NS1. Both these diagnostic modalities were also compared with RT-PCR in 40 acute samples. NS1 detected additional 15 samples, which were missed by PCR. NS1 antigen is an early diagnostic marker that is feasible in a routine diagnostic laboratory.
酶联免疫吸附试验（ELISA）检测特异性 IgM 抗体是诊断登革热感染的主要依据。然而，IgM 抗体在感染后 4 至 5 天产生。早期诊断方法包括病毒分离和逆转录聚合酶链反应（RT-PCR），但这些方法需要复杂的实验室。最近出现的另一种替代方法是 NS1 抗原检测。本研究比较了 87 例急性登革热患者的 IgM 抗体检测与 NS1 抗原检测。NS1 抗原的检测敏感性较高（71-100%），可在发热后第 3 天检测到，而 IgM 在此时的敏感性为 0%至 50%。在发病第 4 天，两种检测方法的敏感性相当。第 4 天以后，IgM 抗体检测优于 NS1 抗原检测。在 40 例急性样本中，还将这两种诊断方法与 RT-PCR 进行了比较。NS1 检测到了另外 15 个 PCR 漏检的样本。NS1 抗原是一种早期诊断标志物，在常规诊断实验室中可行。
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