干扰素可增强牛主动脉内皮细胞中的磷脂酶A2活性。

Interferon enhances phospholipase A2 activity in bovine aortic endothelial cells.

作者信息

Yedgar S, Vlodavsky I, Panet A, Reisfeld N, Eldor A

机构信息

Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

出版信息

Eicosanoids. 1990;3(4):225-9.

PMID:2073400

Abstract

The effect of human interferon-alpha (IFN) on the activity of PLA2 in bovine aortic endothelial cells (BAEC) was studied. A novel method for determination of PLA2 activity in intact cell membranes with a fluorescent analogue of phosphatidylcholine, was employed. It was found that pretreatment of BAEC with human leukocyte IFN alpha or recombinant human IFN alpha 2 enhanced PLA2 activity as a function of IFN concentration. Since PLA2 activity is responsive to the lipid composition of its environment, we analyzed the fatty acyl chain composition of the cell lipids after treatment with IFN. It was found that both IFN alpha and IFN alpha 2 markedly increased the content of arachidonic acid. We suggest that exposure of BAEC to IFN enhances the activity of the cell membrane PLA2 concomitantly with enrichment of the cell membrane lipids with arachidonic acid.

摘要

研究了人α-干扰素（IFN）对牛主动脉内皮细胞（BAEC）中磷脂酶A2（PLA2）活性的影响。采用了一种用磷脂酰胆碱荧光类似物测定完整细胞膜中PLA2活性的新方法。发现用人白细胞α-干扰素或重组人α-2干扰素预处理BAEC可增强PLA2活性，且该活性是IFN浓度的函数。由于PLA2活性对其周围环境的脂质组成有反应，因此我们分析了用IFN处理后细胞脂质的脂肪酸酰基链组成。发现α-干扰素和α-2干扰素均显著增加了花生四烯酸的含量。我们认为，BAEC暴露于IFN会增强细胞膜PLA2的活性，同时使细胞膜脂质富含花生四烯酸。