Kretsch Thomas
Freiburg im Breisgau, Baden-Württemberg, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2010;655:189-202. doi: 10.1007/978-1-60761-765-5_13.
Light is one of the most important exogenous factors regulating plant development throughout the entire life cycle. Light is involved in the breaking of seed dormancy, the regulation of photomorphogenic seedling development, the adaptation of plant morphology toward spectral composition of incident light, and the transition to flowering. Plants have evolved with several photoreceptor families that sense UV-A, blue, red, and far-red light. Here, basal methods to measure light-regulated changes in plant morphology and pigment accumulation will be described. The methods include the determination of apical hook angle and cotyledon opening, the measurement of stem elongation, the determination of leaf surface area, the measurements that characterize light-controlled transition to flowering, and the determination of anthocyanin and chlorophyll accumulation. Furthermore, different light programs are listed that can be used to test for the functional involvement of separate light response modes controlling photomorphogenic plant development.
光是调节植物整个生命周期发育的最重要的外源因素之一。光参与种子休眠的打破、光形态建成的幼苗发育调节、植物形态对入射光光谱组成的适应以及向开花的转变。植物已经进化出几个光感受器家族来感知紫外-A光、蓝光、红光和远红光。在此，将描述测量光调节的植物形态变化和色素积累的基本方法。这些方法包括顶端弯钩角度和子叶张开度的测定、茎伸长的测量、叶表面积的测定、表征光控开花转变的测量以及花青素和叶绿素积累的测定。此外，还列出了不同的光照程序，可用于测试控制光形态建成植物发育的不同光反应模式的功能参与情况。
