体内合成 meganuclease 用于生成转基因斑马鱼 Danio rerio。
In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio.
机构信息
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg-Munich, Germany.
出版信息
J Fish Biol. 2009 Feb;74(2):452-7. doi: 10.1111/j.1095-8649.2008.02075.x.
Abstract
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
摘要
作者表明，在单细胞期共注射编码核靶向的 meganuclease I-SceI 的 mRNA 以及侧翼带有同源限制位点的表达盒，可导致斑马鱼 Danio rerio 中高效稳定的转基因。
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