糖尿病对急性高血压期间血脑屏障破坏的影响。

Effect of diabetes mellitus on disruption of the blood-brain barrier during acute hypertension.

作者信息

Mayhan W G

机构信息

Department of Physiology, University of Nebraska Medical Center, Omaha 68198.

出版信息

Brain Res. 1990 Nov 26;534(1-2):106-10. doi: 10.1016/0006-8993(90)90118-u.

DOI:10.1016/0006-8993(90)90118-u

PMID:2073577

Abstract

The goal of this study was to determine whether the susceptibility of the blood-brain barrier to disruption during acute hypertension is altered during diabetes mellitus. Intravital fluorescent microscopy and fluorescein-labelled albumin were used to evaluate disruption of the blood-brain barrier under control conditions and during acute arterial hypertension in non-diabetic and diabetic rats. Permeability of the blood-brain barrier was quantitated by calculating clearance of fluorescent-labelled albumin and by counting the number of microvascular leaky sites under control conditions and during acute hypertension. In non-diabetic rats, when systemic arterial pressure was increased from 109 +/- 5 (mean +/- S.E.) to 185 +/- 8 mmHg, clearance of albumin increased from 0.16 +/- 0.04 to 1.51 +/- 0.34 ml/s x 10(-6). In diabetic rats, when systemic arterial pressure was increased from 117 +/- 3 to 184 +/- 3 mmHg, clearance of albumin increased from 0.22 +/- 0.10 to 1.30 +/- 0.32 ml/s x 10(-6). These findings suggest that the susceptibility of the blood-brain barrier to disruption during acute hypertension is not altered during diabetes mellitus.

摘要

本研究的目的是确定在糖尿病期间，急性高血压时血脑屏障被破坏的易感性是否发生改变。利用活体荧光显微镜和荧光素标记的白蛋白，在对照条件下以及非糖尿病和糖尿病大鼠急性动脉高血压期间评估血脑屏障的破坏情况。通过计算荧光标记白蛋白的清除率以及在对照条件下和急性高血压期间计数微血管渗漏部位的数量，对血脑屏障的通透性进行定量。在非糖尿病大鼠中，当全身动脉压从109±5（平均值±标准误）升高至185±8 mmHg时，白蛋白清除率从0.16±0.04增加至1.51±0.34 ml/s×10⁻⁶。在糖尿病大鼠中，当全身动脉压从117±3升高至184±3 mmHg时，白蛋白清除率从0.22±0.10增加至1.30±0.32 ml/s×10⁻⁶。这些发现表明，在糖尿病期间，急性高血压时血脑屏障被破坏的易感性未发生改变。