Divisão de Biomateriais, INEB-Instituto de Engenharia Biomédica, Rua do Campo Alegre 823, Porto 4150-180, Portugal.
J Biomed Mater Res A. 2010 Oct;95(1):1-8. doi: 10.1002/jbm.a.32600.
This study concerns the preparation and in vitro characterization of functionalized hydroxyapatite (HA) porous scaffolds, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite scaffolds were prepared using the polymer replication method, and, after being submitted to a specific sintering cycle, collagen Type I was incorporated on the surface. After the coating procedure, collagen was crosslinked using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) conjugation method. In this study, hydroxyapatite scaffolds with uncrosslinked and crosslinked Type I collagen were evaluated. Cell morphology and deposition of extracellular matrix were assessed by scanning electron microscopy, whereas cell distribution was visualized by means of methylene blue staining. MTS and total DNA quantification assays were used to evaluate the viability and proliferation of human bone marrow cells cultured on all the materials for 28 days. Results showed that the cells were able to adhere, proliferate, and form a mineralized matrix on the surface of all the materials. Furthermore, the cells were able to spread from one pore to another and form cell clusters. The results show that these scaffolds are good candidates to serve as drug delivery vehicles and for tissue engineering purposes.
本研究涉及功能化羟基磷灰石（HA）多孔支架的制备和体外特性分析，这些支架旨在用作药物输送系统和骨再生基质。使用聚合物复制法制备羟基磷灰石支架，经过特定的烧结循环后，在表面引入 I 型胶原蛋白。在涂层过程后，使用 N-(3-二甲氨基丙基)-N'-乙基碳二亚胺盐酸盐（EDC）和 N-羟基琥珀酰亚胺（NHS）偶联法对胶原蛋白进行交联。在本研究中，评估了未交联和交联的 I 型胶原的羟基磷灰石支架。通过扫描电子显微镜评估细胞形态和细胞外基质的沉积情况，而通过亚甲蓝染色可视化细胞分布。通过 MTS 和总 DNA 定量测定法评估人骨髓细胞在所有材料上培养 28 天的活力和增殖情况。结果表明，细胞能够在所有材料的表面黏附、增殖和形成矿化基质。此外，细胞能够从一个孔扩散到另一个孔并形成细胞簇。结果表明，这些支架是用作药物输送载体和组织工程的良好候选材料。
