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通过电缆分析测定小管状结构中的短路电流。

Determination of short-circuit current in small tubular structures via cable analysis.

作者信息

Haag K, Knauf H

出版信息

Methods Enzymol. 1990;192:15-26. doi: 10.1016/0076-6879(90)92060-q.

DOI:10.1016/0076-6879(90)92060-q
PMID:2074788
Abstract

Use of cable analysis is a time-consuming maneuver. On the other hand, the advantage of the cable method consists in obtaining the Isc and Rm related to unit area without the explicit measurement of inside radius r of the tubular structure. Obviously, application of the clamping technique requires, in addition, the determination of the surface area. In summary, for small tubular structures, such as the salivary ducts of rats and rabbits, and for human experiments, cable analysis is the method of choice for the Isc determination in vivo as well as in vitro. For larger tubular structures such as the rat colon the Isc should be determined in vivo by the clamping technique described above, whereas the in vitro measurements should be done in an Ussing-type chamber. In the intermediate range of size both in vivo techniques should be applied, in which case one method may serve as a check of the other.

摘要

使用电缆分析是一种耗时的操作。另一方面,电缆法的优点在于无需明确测量管状结构的内半径r即可获得与单位面积相关的短路电流(Isc)和膜电阻(Rm)。显然,应用钳夹技术还需要确定表面积。总之,对于小管状结构,如大鼠和兔子的唾液腺导管,以及人体实验,电缆分析是体内和体外测定Isc的首选方法。对于较大的管状结构,如大鼠结肠,应通过上述钳夹技术在体内测定Isc,而体外测量应在尤斯灌流室中进行。在中等大小范围内,两种体内技术都应应用,在这种情况下,一种方法可作为另一种方法的检验。

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