Reiss K, Kajstura J, Korohoda W
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków/Poland.
Eur J Cell Biol. 1990 Oct;53(1):42-7.
Insulin pulse treatment, lasting for 1 to 3 min, stimulates differentiation of chick embryo myoblasts to myotubes and myofibers in a serum-free medium. The use of serum-free medium supplemented with 2,2'-thiodiethanol permits terminal differentiation of chick embryo myoblasts to cross-striated, spontaneously contracting myofibers. The experiments carried out showed that incubation of chick embryo myoblasts after the insulin pulse treatment for 10 days at 2 degrees C does not inhibit the progress of their differentiation. The data demonstrate that differentiation can be interrupted at any time by transferring cells to 2 degrees C and resumed without delay after returning to 37 degrees C.
胰岛素脉冲处理持续1至3分钟，可在无血清培养基中刺激鸡胚成肌细胞分化为肌管和肌纤维。使用添加了2,2'-硫代二乙醇的无血清培养基可使鸡胚成肌细胞终末分化为横纹肌、能自发收缩的肌纤维。所进行的实验表明，胰岛素脉冲处理后的鸡胚成肌细胞在2℃孵育10天不会抑制其分化进程。数据表明，通过将细胞转移至2℃，分化可在任何时间中断，并在恢复至37℃后立即恢复。
