从含有猴病毒40 DNA片段的易感猴细胞中拯救传染性病毒。
Rescue of infectious virus from permissive monkey cells containing simian virus 40 DNA fragments.
作者信息
Moyer R C, Moyer M P, Gerodetti M H
出版信息
J Virol. 1978 May;26(2):272-80. doi: 10.1128/JVI.26.2.272-280.1978.
Permissive TC7 cells were separately transfected with simian virus 40 (SV40) EcoRI/Hap II A (74% genome) DNA fragments and EcoRI/Hap II B (26% genome) DNA fragments in the presence of DEAE-dextran. Fusion of the progeny of recipient cells receiving the A fragment, TC7 (SV40/74) cells, with TC7 (SV40/26) cells, which had received the B fragment, resulted in SV40 rescue. TC7 (SV40/74 + 26) cells, which had simultaneously received both complementary subgenomes, either spontaneously produced SV40 upon subculture or yielded virus upon treatment with iododeoxyuridine. In addition, fusion of rat cells containing the EcoRI/Hap II A fragment with TC7 (SV40/26) cells resulted in SV40 rescue. Cytopathology, V-antigen production, neutralization, and electron microscopy were parameters used to verify that the rescued virus was SV40. No infectious virus was produced when the combinations of cells fused did not total a complete SV40 genome equivalent.
在存在二乙氨基乙基葡聚糖（DEAE - 葡聚糖）的情况下，将允许性TC7细胞分别用猴病毒40（SV40）EcoRI/Hap II A（74%基因组）DNA片段和EcoRI/Hap II B（26%基因组）DNA片段进行转染。接受A片段的受体细胞的子代，即TC7（SV40/74）细胞，与接受B片段的TC7（SV40/26）细胞融合，导致SV40拯救。同时接受两个互补亚基因组的TC7（SV40/74 + 26）细胞，在传代培养时要么自发产生SV40，要么在用碘脱氧尿苷处理后产生病毒。此外，含有EcoRI/Hap II A片段的大鼠细胞与TC7（SV40/26）细胞融合导致SV40拯救。细胞病理学、V抗原产生、中和以及电子显微镜检查是用于验证拯救出的病毒为SV40的参数。当融合的细胞组合不构成完整的SV40基因组当量时，不产生感染性病毒。
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