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[表达蒺藜苜蓿鸟氨酸转氨酶cDNA的烟草转化体]

[Tobacco transformants expressing the Medicago truncatula ornithine aminotransferase cDNA].

作者信息

Gerasimova S V, Kolodiazhnaia Ia S, Titov S E, Romanova A V, Koval' V S, Kochetov A V, Shumnyĭ V K

出版信息

Genetika. 2010 Jul;46(7):1000-3.

Abstract

The Medicago truncatula ornithine aminotransferase cDNA was cloned under the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into the genome of tobacco Nicotiana tabacum SR1 plants. Transformed tobacco plants grew better in salinity stress, but did not differ in proline content under normal or stress conditions from control plants. It was assumed that the role of ornithine aminotransferase in the molecular mechanisms of stress resistance is not associated with additional proline synthesis.

摘要

蒺藜苜蓿鸟氨酸转氨酶cDNA在花椰菜花叶病毒强大的组成型35S RNA启动子控制下被克隆,并转入烟草Nicotiana tabacum SR1植株的基因组中。转化后的烟草植株在盐胁迫下生长得更好,但在正常或胁迫条件下,其脯氨酸含量与对照植株并无差异。据推测,鸟氨酸转氨酶在抗逆分子机制中的作用与额外的脯氨酸合成无关。

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