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堪察加半岛虹鳟(副鲑属(大麻哈鱼属)虹鳟)微卫星DNA分析:基因座选择与方法优化

[Analysis of microsatellite DNA of rainbow trout (Parasalmo (Oncorhynchus) mykiss) of Kamchatka: selection of loci and optimization of the method].

作者信息

Semenova A V, Rubtsova G A, Afanas'ev K I, Pavlov S D

出版信息

Genetika. 2010 Jul;46(7):1004-8.

Abstract

The variation of a sample of rainbow trout (Parasalmo (Oncorhynchus) mykiss) from natural populations of several rivers of the Kamchatka Peninsula with respect to 43 microsatellite DNA loci has been studied. These loci were earlier used for analysis of Asian populations of closely related salmonids. Ten of them may be regarded as markers and seen promising for further studies on intraspecific relationships of rainbow trout of Kamchatka. Their use in studies on more numerous samples from different localities and populations of Parasalmo (O.) mykiss in the Asian part of the species range will ensure efficient population genetic analysis of the Kamchatka population group of this species.

摘要

对堪察加半岛多条河流自然种群中的虹鳟(Parasalmo (Oncorhynchus) mykiss)样本在43个微卫星DNA位点上的变异情况进行了研究。这些位点此前用于分析亲缘关系密切的鲑科鱼类的亚洲种群。其中10个位点可被视为标记,有望用于堪察加虹鳟种内关系的进一步研究。在该物种分布范围亚洲部分,将这些位点用于对来自不同地点和种群的更多Parasalmo (O.) mykiss样本的研究,将确保对该物种堪察加种群组进行有效的群体遗传学分析。

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