RNA 中的 G-四链体结构可刺激线粒体转录终止和引物形成。
G-quadruplex structures in RNA stimulate mitochondrial transcription termination and primer formation.
机构信息
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, SE-405 30 Gothenborg, Sweden.
出版信息
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16072-7. doi: 10.1073/pnas.1006026107. Epub 2010 Aug 26.
Abstract
The human mitochondrial transcription machinery generates the primers required for initiation of leading-strand DNA replication. According to one model, the 3' end of the primer is defined by transcription termination at conserved sequence block II (CSB II) in the mitochondrial DNA control region. We here demonstrate that this site-specific termination event is caused by G-quadruplex structures formed in nascent RNA upon transcription of CSB II. We also demonstrate that a poly-dT stretch downstream of CSB II has a modest stimulatory effect on the termination efficiency. The mechanism is reminiscent of Rho-independent transcription termination in prokaryotes, with the exception that a G-quadruplex structure replaces the hairpin loop formed in bacterial mRNA during transcription of terminator sequences.
摘要
人类线粒体转录机制生成启动领头链 DNA 复制所需的引物。根据一种模型，引物的 3' 端由线粒体 DNA 控制区中保守序列块 II (CSB II) 的转录终止来定义。我们在此证明，这种位点特异性终止事件是由 CSB II 转录时新生 RNA 中形成的 G-四链体结构引起的。我们还证明，CSB II 下游的多聚 dT 延伸对终止效率有适度的刺激作用。该机制让人联想到原核生物中无 Rho 依赖的转录终止，不同之处在于，在终止序列的转录过程中，细菌 mRNA 中形成的发夹环被 G-四链体结构取代。
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