Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, Manitoba, Canada.
Ann Anat. 2010 Sep 20;192(5):292-301. doi: 10.1016/j.aanat.2010.07.007. Epub 2010 Aug 6.
Tumorigenesis includes alterations in the three-dimensional (3D) nuclear organization of the genome. The combination of sensitive quantitative fluorescent in situ hybridization (Q-FISH) and three-dimensional (3D) microscopy have evolved as powerful tools in studying the dynamic 3D organization of telomeres and chromosomes in the interphase nucleus of individual normal and tumor cells. Tumor-specific alterations in 3D telomere architecture, particularly the appearance of telomeric aggregates, are early events in tumorigenesis and have diagnostic and prognostic value. Novel tools in the 3D nuclear imaging arsenal now include high-throughput scanning capabilities and new 3D nano-resolution microscopy of tissues and cells. In this review, we summarize our current understanding of the biology of telomeres in the context of tumorigenesis and elucidate the important integrating function of advanced 3D imaging technologies in translating new discoveries in basic cancer research into new diagnostic tools for clinical oncologists to improve patient care.
肿瘤发生包括基因组三维（3D）核组织的改变。敏感的定量荧光原位杂交（Q-FISH）和三维（3D）显微镜的结合已经发展成为研究正常和肿瘤细胞间期核中端粒和染色体的动态 3D 组织的有力工具。3D 端粒结构的肿瘤特异性改变，特别是端粒聚集的出现，是肿瘤发生的早期事件，具有诊断和预后价值。3D 核成像武器库中的新工具包括高通量扫描功能和组织和细胞的新型 3D 纳米分辨率显微镜。在这篇综述中，我们总结了我们目前对端粒在肿瘤发生中的生物学的理解，并阐明了先进的 3D 成像技术在将基础癌症研究中的新发现转化为临床肿瘤学家的新诊断工具以改善患者治疗方面的重要综合功能。
