Vanderzwalmen P, Zech N, Lejeune B, Wirtleitner B, Zech M, Ectors F
IVF Centers Prof. Zech, Bregenz, Autriche.
Gynecol Obstet Fertil. 2010 Sep;38(9):536-40. doi: 10.1016/j.gyobfe.2010.07.008. Epub 2010 Aug 25.
The use of high levels of cryoprotectants (CPs) in solutions applied to vitrify oocytes or embryos is an argument to still prefer slow freezing procedure. Is it a justified argument? Out of three studies using mice zygotes we may assume that (i) the intracellular concentration of CPs is far lower than the one in the vitrification solutions, (ii) the intracellular concentration of CPs in the vitrified zygote is in contrary to the common beliefs even lower than the one observed after a slow freezing procedure, (iii) survival after slow freezing reflects the presence of an intracellular vitrified state in these cells.
在用于玻璃化冷冻卵母细胞或胚胎的溶液中使用高浓度的冷冻保护剂（CPs），这成为仍然更倾向于慢速冷冻程序的一个理由。这个理由合理吗？在三项使用小鼠受精卵的研究中，我们可以假定：（i）CPs的细胞内浓度远低于玻璃化溶液中的浓度；（ii）与普遍看法相反，玻璃化受精卵中的CPs细胞内浓度甚至低于慢速冷冻程序后观察到的浓度；（iii）慢速冷冻后的存活率反映了这些细胞中存在细胞内玻璃化状态。
