Trace Analysis and Biosensor Research Center, Center for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Biosens Bioelectron. 2010 Oct 15;26(2):357-64. doi: 10.1016/j.bios.2010.08.005. Epub 2010 Aug 7.
Boronic acid that can reversibly bind to diols was used to detect bacteria through its affinity binding reaction with diol-groups on bacterial cell walls. 3-aminophenylboronic acid (3-APBA) was immobilized on a gold electrode via a self-assembled monolayer. The change in capacitance of the sensing surface caused by the binding between 3-APBA and bacteria in a flow system was detected by a potentiostatic step method. Under optimal conditions the linear range of 1.5×10(2)-1.5×10(6) CFU ml(-1) and the detection limit of 1.0×10(2) CFU ml(-1) was obtained. The sensing surface can be regenerated and reused up to 58 times. The method was used for the analysis of bacteria in several types of water, i.e., bottled, well, tap, reservoir and wastewater. Compared with the standard plate count method, the results were within one standard deviation of each other. The proposed method can save both time and cost of analysis. The electrode modified with 3-APBA would also be applicable to the detection of other cis-diol-containing analytes. The concept could be extended to other chemoselective ligands, offering less expensive and more robust affinity sensors for a wide range of compounds.
硼酸可以与二醇可逆结合，通过硼酸与细胞壁上二醇基团的亲和结合反应来检测细菌。3-氨苯基硼酸（3-APBA）通过自组装单层固定在金电极上。通过恒电位阶跃法检测流动系统中 3-APBA 与细菌之间结合引起的传感表面电容变化。在最佳条件下，得到了 1.5×10(2)-1.5×10(6) CFU ml(-1) 的线性范围和 1.0×10(2) CFU ml(-1) 的检测限。传感表面可重复使用多达 58 次。该方法用于分析几种类型的水，即瓶装水、井水、自来水、水库水和废水。与标准平板计数法相比，结果彼此相差一个标准差。该方法可以节省分析的时间和成本。修饰有 3-APBA 的电极也可用于检测其他含有顺二醇的分析物。该概念可以扩展到其他化学选择性配体，为广泛的化合物提供更经济、更稳健的亲和传感器。
