通过包含异源 recA 来增强山羊支原体亚种的靶向同源重组。
Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.
机构信息
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
出版信息
Appl Environ Microbiol. 2010 Oct;76(20):6951-4. doi: 10.1128/AEM.00056-10. Epub 2010 Aug 27.
Abstract
A suicide plasmid, pExp1-ctpA::tetM-recAec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.
摘要
使用自杀质粒 pExp1-ctpA::tetM-recAec，该质粒携带来自大肠杆菌的 recA 和 tetM 作为选择标记，通过靶向基因敲除，在山羊支原体亚种中生成了 ctpA 敲除突变体。在同源重组过程中，大肠杆菌 recA 的存在极大地提高了突变体的生成一致性和回收效率。
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