脓肿分枝杆菌的基因操作
Genetic manipulation of Mycobacterium abscessus.
作者信息
Medjahed Halima, Singh Anil Kumar
机构信息
Université Paris Descartes, Faculté de Médecine Paris Descartes, Paris, France.
出版信息
Curr Protoc Microbiol. 2010 Aug;Chapter 10:Unit 10D.2. doi: 10.1002/9780471729259.mc10d02s18.
Abstract
This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.
摘要
本单元涵盖了脓肿分枝杆菌的基因操作方案，包括核酸提取（质粒DNA、基因组DNA和RNA）、转化以及一种重组工程诱变方法。脓肿分枝杆菌是一种生物安全2级（BSL-2）细菌，同时也讨论了操作注意事项。
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