State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai, China.
Biotechnol Bioeng. 2011 Jan;108(1):216-21. doi: 10.1002/bit.22915.
Intracellular signals are critical to secondary metabolite biosynthesis by plant cells, but their quantitative effects are not yet well understood in plant cell cultures. Using Taxus chinensis suspension culture, which has the potential to provide a sustainable supply of highly useful bioactive taxoids, this work investigated the impact of endogenous salicylic acid (SA) level on taxuyunnanine C (Tc) biosynthesis. It was observed that the Tc accumulation was strongly dependent on the endogenous SA level. Under elicitation with an endogenous SA of 97.1 ± 11.8 µg/g dry weight, a maximal Tc content of 10.3 ± 0.52 mg/g dry weight was obtained. The transcription levels of Tc biosynthetic genes of geranylgeranyl diphosphate synthase and taxadiene synthase were also analyzed, and they were increased with increase of internal SA level. The results demonstrated that manipulation of endogenous SA level could be an efficient strategy for improving secondary metabolite biosynthesis in plant cell culture.
细胞内信号对植物细胞次生代谢产物的生物合成至关重要，但在植物细胞培养中，它们的定量影响尚不清楚。本研究以具有提供高价值生物活性紫杉烷可持续供应潜力的中国红豆杉悬浮培养物为材料，探讨了内源性水杨酸（SA）水平对紫杉烷 C（Tc）生物合成的影响。结果表明，Tc 的积累强烈依赖于内源性 SA 水平。在以 97.1 ± 11.8 µg/g 干重的内源性 SA 诱导下，获得了 10.3 ± 0.52 mg/g 干重的最大 Tc 含量。还分析了 Tc 生物合成基因香叶基二磷酸合酶和紫杉烯合酶的转录水平，它们随内部 SA 水平的增加而增加。结果表明，内源性 SA 水平的调控可能是提高植物细胞培养物次生代谢产物生物合成的有效策略。
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