Holland G R, Robinson P P
Division of Endodontics, University of Alberta, Edmonton, Canada.
J Anat. 1990 Aug;171:179-86.
This investigation was designed to determine the change in cell numbers in the trigeminal ganglia following unilateral section of one of its peripheral branches. In 9 young adult cats, under general anesthesia, the inferior alveolar nerve was transected. In 3 of the animals the cut ends were reapposed and in the other 6 regeneration was blocked. After 15 weeks the trigeminal ganglia were removed, and the number of neurons present estimated by counting nucleoli in every third section. The counts were compared with those obtained from 3 unoperated control animals. The mean number of cells in the ganglia of control animals was 14,324 with no statistically significant difference between sides. There was no significant difference between counts from opposite sides of cats whose nerves were allowed to regenerate. In the animals in which regeneration was prevented the mean count on the operated side was 13,874 and on the unoperated 18,374. These differences were statistically significant and appeared to result from an increase in cell counts on the unoperated side rather than a reduction in the counts on the operated side. This may be explained by the presence of neurons with inconspicuous nucleoli in normal ganglia, which are stimulated to enlarge and become more prominent following peripheral nerve injury. This change could occur on both sides and would mask cell loss on the operated side and produce an apparent increase in the count on the unoperated side.
本研究旨在确定三叉神经节外周分支之一被单侧切断后细胞数量的变化。在9只成年幼猫中，在全身麻醉下切断下牙槽神经。其中3只动物的切断端重新对接，另外6只动物的神经再生被阻断。15周后取出三叉神经节，通过计算每第三切片中的核仁数量来估计神经元数量。将这些计数结果与3只未手术的对照动物的结果进行比较。对照动物神经节中的细胞平均数量为14,324个，两侧之间无统计学显著差异。神经被允许再生的猫的两侧计数之间也无显著差异。在再生被阻止的动物中，手术侧的平均计数为13,874个，未手术侧为18,374个。这些差异具有统计学显著性，似乎是由于未手术侧的细胞计数增加，而不是手术侧的计数减少。这可能是因为正常神经节中存在核仁不明显的神经元，在周围神经损伤后它们受到刺激而增大并变得更加明显。这种变化可能在两侧都发生，会掩盖手术侧的细胞损失，并导致未手术侧的计数明显增加。
