通过对映选择性亚胺还原酶的细菌全细胞催化剂，从环状亚胺不对称合成手性环状胺。
Asymmetric synthesis of chiral cyclic amine from cyclic imine by bacterial whole-cell catalyst of enantioselective imine reductase.
机构信息
Department of Biomolecular Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
出版信息
Org Biomol Chem. 2010 Oct 21;8(20):4533-5. doi: 10.1039/c0ob00353k. Epub 2010 Aug 31.
PMID:20820664
Abstract
Streptomyces sp. GF3587 and 3546 were found to be imine-reducing strains with high R- and S-selectivity by screening using 2-methyl-1-pyrroline (2-MPN). Their whole-cell catalysts produced 91 mM R-2-methylpyrrolidine (R-2-MP) with 99.2%e.e. and 27.5 mM S-2-MP (92.3%e.e.) from 2-MPN at 91-92% conversion in the presence of glucose, respectively.
摘要
通过使用 2-甲基-1-吡咯啉（2-MPN）筛选，发现链霉菌 GF3587 和 3546 是具有高 R-和 S-选择性的亚胺还原菌株。它们的全细胞催化剂在葡萄糖存在下，分别将 2-MPN 转化为 91-92%，生成 91mM 的 R-2-甲基吡咯烷（R-2-MP），ee 值为 99.2%，生成 27.5mM 的 S-2-MP（ee 值为 92.3%）。
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