可可树（锦葵科）帕里纳里系列幼苗的基因图谱，致死基因黄体-Pa的携带者

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa.

作者信息

Rehem B C, Almeida A-A F, Corrêa R X, Gesteira A S, Yamada M M, Valle R R

机构信息

Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.

出版信息

Genet Mol Res. 2010 Sep 8;9(3):1775-84. doi: 10.4238/vol9-3gmr889.

DOI:10.4238/vol9-3gmr889

PMID:20830669

Abstract

The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 SSR and 64 RAPD primers. The SSR primers amplified 65 RAPD primers, giving 179 polymorphic bands. After screening with SSR and RAPD markers, we selected 20 SSR primers, two SSR primers with ESTs and 22 RAPD primers that were polymorphic for genitors Pa 30 and Pa 169. Only two of the 22 RAPD primers and three of the 20 SSR primers were informative and polymorphic in the analysis of the bulk samples of progenies. Among these, primer RAPD E11 produced a band linked to the lethal gene (38.5 cM); none of the SSRs were associated with 'Luteus-Pa'.

摘要

致死基因“Luteus-Pa”存在于来自秘鲁的帕里纳里（Pa）系列可可基因型（可可树）中。受该基因影响的幼苗叶片发黄，随后死亡。我们基于微卫星标记和随机扩增多态性DNA（RAPD）对该基因进行定位，以阐明“Luteus-Pa”的遗传方式并探究可能的致死机制。用87个简单序列重复（SSR）引物和64个RAPD引物对亲本的DNA样本进行扩增。SSR引物扩增出65个RAPD引物，产生了179条多态性条带。在用SSR和RAPD标记进行筛选后，我们选择了20个SSR引物、两个带有表达序列标签（EST）的SSR引物和22个对亲本Pa 30和Pa 169具有多态性的RAPD引物。在对后代的混合样本分析中，22个RAPD引物中只有两个、20个SSR引物中只有三个具有信息性且呈多态性。其中，RAPD引物E11产生了一条与致死基因连锁的条带（38.5厘摩）；没有SSR与“Luteus-Pa”相关联。