Key Laboratory for Special Functional Aggregated Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, People's Republic of China.
Appl Biochem Biotechnol. 2011 Mar;163(6):744-55. doi: 10.1007/s12010-010-9079-9. Epub 2010 Sep 12.
The extraction of bovine serum albumin (BSA) has been investigated using reverse micelles of hexadecyl trimethyl ammonium chloride/n-octanol/isooctane. Forward extraction process parameters such as the surfactant concentration, co-solvent concentration, pH, ionic strength, and species of the initial aqueous phase were important factors affecting the extraction performance. These parameters were varied to optimize the extraction efficiency. Under the optimized conditions, forward extraction efficiencies of BSA can reach practically 99.55%. The thermodynamic study revealed that the extraction of BSA is controlled by entropy changes. Maximum back-extraction efficiency of 85.16% can be obtained at low pH values and high salt concentrations. The structures of BSA during reverse micelle extraction did not change by comparing the circular dichroism spectra of BSA back-extracted to the aqueous phase with that of feed BSA.
牛血清白蛋白（BSA）的萃取采用十六烷基三甲基氯化铵/正辛醇/异辛烷反胶束进行研究。正向萃取过程中的参数，如表面活性剂浓度、共溶剂浓度、pH 值、离子强度和初始水相的种类，是影响萃取性能的重要因素。这些参数的变化可以优化萃取效率。在优化条件下，BSA 的正向萃取效率可达 99.55%。热力学研究表明，BSA 的萃取受熵变控制。在低 pH 值和高盐浓度下，可以获得最大的反萃取效率 85.16%。通过比较反胶束萃取后 BSA 的圆二色光谱与进料 BSA 的圆二色光谱，发现 BSA 的结构在反胶束萃取过程中没有发生变化。
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