成纤维细胞生长因子受体 4 Arg388 等位基因与胃癌进展相关。
The fibroblast growth factor receptor-4 Arg388 allele is associated with gastric cancer progression.
机构信息
Department of Abdominal Surgery, Shanghai Cancer Center, Fu Dan University, Shanghai, China.
出版信息
Ann Surg Oncol. 2010 Dec;17(12):3354-61. doi: 10.1245/s10434-010-1323-6. Epub 2010 Sep 16.
BACKGROUND
Fibroblast growth factor receptor 4 (FGFR4) Gly388Arg polymorphism, located in the FGFR4 gene exon 9, was reported to be associated with malignant tumors prognosis; however, there has been no relevant research for gastric cancer. The purpose of this study was to investigate the clinical significance of FGFR4 Gly388Arg polymorphism as well as the mRNA expression of FGFR4 in patients with gastric cancer.
METHODS
The mRNA expression of FGFR4 in 103 gastric cancer tissues and corresponding normal tissues were measured by reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. PCR-restriction fragment length polymorphism analysis was performed to detect the FGFR4 Gly388Arg in 103 gastric cancer tissues.
RESULTS
In 57.3% of patients, homozygous or heterozygous Arg388 allele was present. FGFR4 expressions in mRNA levels were higher in gastric cancer tissues compared with those in relevant normal tissues. However, there is no significantly statistical difference compared with mRNA expression of FGFR4 in different genotypes. Associations between FGFR4 Gly388Arg polymorphism and overall survival exist in patients with gastric cancer (P = 0.046).The FGFR4 Arg allele (hazard risk (HR), 2.324; 95% confidence interval (CI), 1.054-4.125; P = 0.037) and TNM stage (HR, 5.516; 95% CI 3.658-7.409; P = 0.005) were independent prognostic factors in patients with gastric cancer.
CONCLUSIONS
Based on this study, FGFR4 Arg388 genotype-a marker for gastric cancer progression-may predict prognosis of gastric cancer.
背景
成纤维细胞生长因子受体 4（FGFR4）Gly388Arg 多态性位于 FGFR4 基因外显子 9 中，据报道与恶性肿瘤的预后有关；然而，目前还没有关于胃癌的相关研究。本研究旨在探讨 FGFR4 Gly388Arg 多态性以及 FGFR4 mRNA 表达在胃癌患者中的临床意义。
方法
采用逆转录聚合酶链反应（PCR）和实时定量 PCR 检测 103 例胃癌组织及相应正常组织中 FGFR4 mRNA 的表达。采用 PCR-限制性片段长度多态性分析检测 103 例胃癌组织中 FGFR4 Gly388Arg。
结果
在 57.3%的患者中，存在纯合或杂合 Arg388 等位基因。胃癌组织中 FGFR4 的 mRNA 水平表达高于相应的正常组织，但与不同基因型的 FGFR4 mRNA 表达无显著统计学差异。FGFR4 Gly388Arg 多态性与胃癌患者的总生存期存在相关性（P = 0.046）。FGFR4 Arg 等位基因（危险比（HR），2.324；95%置信区间（CI），1.054-4.125；P = 0.037）和 TNM 分期（HR，5.516；95%CI，3.658-7.409；P = 0.005）是胃癌患者的独立预后因素。
结论
基于本研究，FGFR4 Arg388 基因型——胃癌进展的标志物——可能预测胃癌的预后。
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