Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, China.
J Gene Med. 2010 Oct;12(10):832-9. doi: 10.1002/jgm.1501.
Clinical studies have shown that gene therapy is a promising approach for treating such genetic diseases as the eye disease, Leber's congenital amaurosis. Development of gene therapy approaches for treating chronic inflammatory diseases is, however, more challenging because it requires the production of anti-inflammatory molecules at the diseased tissues only when they are needed.
We designed such a system by modifying the human interleukin (IL)-6 gene promoter to direct transgene expression and delivered the system into cultured cells as well as mouse lungs using a helper-dependent adenoviral vector.
We have demonstrated both in vitro and in vivo that the reporter LacZ or human IL-10 gene can be induced by inflammatory stimuli.
The results obtained indicate that the inflammation inducible gene expression system based on the modified human IL-6 gene promoter has the potential to be used for developing gene therapy for treating inflammatory diseases.
临床研究表明，基因治疗是治疗某些遗传性疾病（如眼疾莱伯先天性黑蒙症）的一种很有前途的方法。然而，开发用于治疗慢性炎症性疾病的基因治疗方法更具挑战性，因为它需要仅在需要时在患病组织中产生抗炎分子。
我们通过修饰人白细胞介素（IL）-6 基因启动子来设计这样的系统，以指导转基因表达，并使用辅助依赖性腺病毒载体将该系统递送至培养的细胞和小鼠肺部。
我们已经在体外和体内证明了报告基因 LacZ 或人白细胞介素 10 基因可以被炎症刺激诱导。
所得结果表明，基于修饰的人白细胞介素 6 基因启动子的炎症诱导基因表达系统有可能用于开发治疗炎症性疾病的基因治疗方法。
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