Biotechnology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Iran.
Arch Med Res. 2010 Jul;41(5):343-9. doi: 10.1016/j.arcmed.2010.06.007.
The overall prevalence of obesity and metabolic syndrome (MetS) is increasing among children and adolescents and can predispose to type II diabetes mellitus and cardiovascular disease. There are reported associations between an angiotensin II type I receptor gene polymorphism (AT(1)R/A1166C) with hypertension, myocardial infarction, insulin resistance and cardiovascular disease risk. In the present study, we aimed to investigate whether the AT(1)R/A1166C polymorphism was associated with MetS among adolescent Iranian girls.
A total of 350 adolescent girls aged 15-17 years from high schools and different educational zones of Mashhad city participated in this population-based, genetic association study. Of these individuals, 101 patients had MetS (defined by the NCEP-ATP III criteria); the remaining 249 age-matched girls were considered as the control group. All subjects were genotyped for the AT(1)R/A1166C polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
Frequencies of the AA, AC and CC genotypes were 164 (65.9%), 80 (32.1%) and 5 (2.0%) in the control group and 79 (78.2%), 20 (19.8%) and 2 (2.0%) in patients, which were not consistent with the Hardy-Weinberg equilibrium (p <0.05 and p <0.001, respectively). Frequency of the AT(1)R C allele was found to be significantly lower in patients compared with controls (p <0.05).
Our findings suggested that the 1166C allele of AT(1)R gene may be associated with a decreased risk of MetS in adolescent Iranian females.
肥胖症和代谢综合征（MetS）在儿童和青少年中的总体患病率正在上升，并且可能导致 2 型糖尿病和心血管疾病。有报道称血管紧张素 II 型 1 型受体基因多态性（AT（1）R/A1166C）与高血压、心肌梗死、胰岛素抵抗和心血管疾病风险之间存在关联。在本研究中，我们旨在研究 AT（1）R/A1166C 多态性是否与伊朗青少年女性的 MetS 有关。
本研究共纳入了来自马什哈德市高中和不同教育区的 350 名 15-17 岁的青少年女孩，这些参与者均来自以人群为基础的遗传关联研究。其中，101 名患者患有 MetS（符合 NCEP-ATP III 标准）；其余 249 名年龄匹配的女孩被视为对照组。所有受试者均采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对 AT（1）R/A1166C 多态性进行基因分型。
在对照组中，AA、AC 和 CC 基因型的频率分别为 164（65.9%）、80（32.1%）和 5（2.0%），而在患者中，这三种基因型的频率分别为 79（78.2%）、20（19.8%）和 2（2.0%），与 Hardy-Weinberg 平衡不一致（分别为 p<0.05 和 p<0.001）。与对照组相比，患者中 AT（1）R C 等位基因的频率显著降低（p<0.05）。
我们的研究结果表明，AT（1）R 基因的 1166C 等位基因可能与伊朗青少年女性 MetS 风险降低有关。
