通过单细胞固氮蓝藻属的单链 DNA 进行遗传转化和诱变。
Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.
机构信息
Purdue University, Department of Biological Sciences, West Lafayette, IN 47907, USA.
出版信息
Appl Environ Microbiol. 2010 Nov;76(22):7641-5. doi: 10.1128/AEM.01456-10. Epub 2010 Sep 17.
Abstract
We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.
摘要
我们描述了一种使用单链 DNA 技术在 Cyanothece sp. 菌株 PCC 7822 中产生特定基因敲除的遗传系统（B. Zorin、P. Hegemann 和 I. Sizova，Eukaryot. Cell 4:1264-1272, 2005）。第一个完全分离的突变体是一个 ΔnifK 突变体，它不能在缺乏复合氮的培养基中生长，几乎不产生氢气。
相似文献
1
Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.
Appl Environ Microbiol. 2010 Nov;76(22):7641-5. doi: 10.1128/AEM.01456-10. Epub 2010 Sep 17.
2
Enhanced Nitrogen Fixation in a -Deficient Strain of sp. Strain ATCC 51142, a Unicellular Nitrogen-Fixing Cyanobacterium.
Appl Environ Microbiol. 2019 Mar 22;85(7). doi: 10.1128/AEM.02887-18. Print 2019 Apr 1.
3
Variations in the rhythms of respiration and nitrogen fixation in members of the unicellular diazotrophic cyanobacterial genus Cyanothece.
Plant Physiol. 2013 Mar;161(3):1334-46. doi: 10.1104/pp.112.208231. Epub 2012 Dec 28.
4
The uptake hydrogenase in the unicellular diazotrophic cyanobacterium Cyanothece sp. strain PCC 7822 protects nitrogenase from oxygen toxicity.
J Bacteriol. 2014 Feb;196(4):840-9. doi: 10.1128/JB.01248-13. Epub 2013 Dec 6.
5
Better living through cyanothece - unicellular diazotrophic cyanobacteria with highly versatile metabolic systems.
Adv Exp Med Biol. 2010;675:275-90. doi: 10.1007/978-1-4419-1528-3_16.
6
The effects of different light-dark cycles on the metabolism of the diazotrophic, unicellular cyanobacteria Cyanothece sp. ATCC 51142, and Cyanothecesp. PCC 7822.
J Phycol. 2014 Oct;50(5):930-8. doi: 10.1111/jpy.12224. Epub 2014 Sep 14.
7
Enzyme kinetics, inhibitors, mutagenesis and electron paramagnetic resonance analysis of dual-affinity nitrate reductase in unicellular N(2)-fixing cyanobacterium Cyanothece sp. PCC 8801.
Plant Physiol Biochem. 2011 Nov;49(11):1369-76. doi: 10.1016/j.plaphy.2011.07.007. Epub 2011 Jul 23.
8
The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle.
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15094-9. doi: 10.1073/pnas.0805418105. Epub 2008 Sep 23.
9
Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes.
Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6156-61. doi: 10.1073/pnas.0711068105. Epub 2008 Apr 21.
10
Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822.
Photosynth Res. 2013 Nov;118(1-2):25-36. doi: 10.1007/s11120-013-9941-z. Epub 2013 Oct 19.
引用本文的文献
1
A Genetic Toolbox for the New Model Cyanobacterium PCC 7425: A Case Study for the Photosynthetic Production of Limonene.
Front Microbiol. 2020 Sep 18;11:586601. doi: 10.3389/fmicb.2020.586601. eCollection 2020.
2
Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.
Microorganisms. 2019 Sep 29;7(10):409. doi: 10.3390/microorganisms7100409.
3
Enhanced Nitrogen Fixation in a -Deficient Strain of sp. Strain ATCC 51142, a Unicellular Nitrogen-Fixing Cyanobacterium.
Appl Environ Microbiol. 2019 Mar 22;85(7). doi: 10.1128/AEM.02887-18. Print 2019 Apr 1.
4
Altering the Structure of Carbohydrate Storage Granules in the Cyanobacterium Synechocystis sp. Strain PCC 6803 through Branching-Enzyme Truncations.
J Bacteriol. 2015 Dec 14;198(4):701-10. doi: 10.1128/JB.00830-15.
5
Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light-dark cycle.
BMC Genomics. 2014 Dec 29;15(1):1185. doi: 10.1186/1471-2164-15-1185.
6
Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth in nonheterocystous cyanobacteria.
Proc Natl Acad Sci U S A. 2014 May 6;111(18):6762-7. doi: 10.1073/pnas.1323570111. Epub 2014 Apr 21.
7
Rapid construction of metabolic models for a family of Cyanobacteria using a multiple source annotation workflow.
BMC Syst Biol. 2013 Dec 27;7:142. doi: 10.1186/1752-0509-7-142.
8
The uptake hydrogenase in the unicellular diazotrophic cyanobacterium Cyanothece sp. strain PCC 7822 protects nitrogenase from oxygen toxicity.
J Bacteriol. 2014 Feb;196(4):840-9. doi: 10.1128/JB.01248-13. Epub 2013 Dec 6.
9
Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822.
Photosynth Res. 2013 Nov;118(1-2):25-36. doi: 10.1007/s11120-013-9941-z. Epub 2013 Oct 19.
10
Diurnal rhythm of a unicellular diazotrophic cyanobacterium under mixotrophic conditions and elevated carbon dioxide.
Photosynth Res. 2013 Nov;118(1-2):51-7. doi: 10.1007/s11120-013-9888-0. Epub 2013 Jul 25.
本文引用的文献
1
TRANSCRIPTIONAL ANALYSIS OF THE UNICELLULAR, DIAZOTROPHIC CYANOBACTERIUM CYANOTHECE SP. ATCC 51142 GROWN UNDER SHORT DAY/NIGHT CYCLES(1).
J Phycol. 2009 Jun;45(3):610-20. doi: 10.1111/j.1529-8817.2009.00674.x. Epub 2009 May 18.
2
Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light.
Appl Environ Microbiol. 2010 Jul;76(13):4293-301. doi: 10.1128/AEM.00146-10. Epub 2010 May 7.
3
The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle.
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15094-9. doi: 10.1073/pnas.0805418105. Epub 2008 Sep 23.
4
Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes.
Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6156-61. doi: 10.1073/pnas.0711068105. Epub 2008 Apr 21.
5
Differential transcriptional analysis of the cyanobacterium Cyanothece sp. strain ATCC 51142 during light-dark and continuous-light growth.
J Bacteriol. 2008 Jun;190(11):3904-13. doi: 10.1128/JB.00206-08. Epub 2008 Apr 4.
6
Winding up the cyanobacterial circadian clock.
Trends Microbiol. 2007 Sep;15(9):381-8. doi: 10.1016/j.tim.2007.08.005. Epub 2007 Sep 4.
7
Breaking the N2 triple bond: insights into the nitrogenase mechanism.
Dalton Trans. 2006 May 21(19):2277-84. doi: 10.1039/b517633f. Epub 2006 Apr 11.
8
Intermediates trapped during nitrogenase reduction of N triple bond N, CH3-N=NH, and H2N-NH2.
J Am Chem Soc. 2005 Nov 2;127(43):14960-1. doi: 10.1021/ja0539342.
9
Nuclear-gene targeting by using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii.
Eukaryot Cell. 2005 Jul;4(7):1264-72. doi: 10.1128/EC.4.7.1264-1272.2005.
10
Substrate interactions with the nitrogenase active site.
Acc Chem Res. 2005 Mar;38(3):208-14. doi: 10.1021/ar040050z.
Zotero 插件