Liu Fen, Zeng Zhen-Guo, Ding Cheng-Zhi, Shao Qiang, Li Yong, Qian Ke-Jian
Intensive Care Unit, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Sep;22(9):540-2.
To investigate the expression of microRNA-146a (miRNA-146a) in NR8383 alveolar macrophages treated with lipopolysaccharides (LPS).
NR8383 alveolar macrophages were divided into two groups: LPS treated group and phosphate buffer saline (PBS) control group, and they cultured for 6 hours. The production of tumor necrosis factor-α (TNF-α) in the supernatant of cells was determined with enzyme-linked immunosorbent assay (ELISA), and the expression of miRNA-146a of cells was detected by real-time polymerase chain reaction (PCR).
Compared with PBS control group, the TNF-α content (ng/L) in LPS treated group was significantly increased (650.26±40.53 vs. 6.23±1.76, P<0.01), and miRNA-146a in LPS treated group increased by about (5.33±0.81) folds (P<0.01).
The expression of miRNA-146a was increased in LPS treated NR8383 cells, and miRNA-146a may be involved in the modulation inflammatory response of the NR8383 alveolar macrophage.
研究脂多糖（LPS）处理的NR8383肺泡巨噬细胞中微小RNA-146a（miRNA-146a）的表达情况。
将NR8383肺泡巨噬细胞分为两组：LPS处理组和磷酸盐缓冲盐水（PBS）对照组，培养6小时。采用酶联免疫吸附测定（ELISA）法测定细胞上清液中肿瘤坏死因子-α（TNF-α）的产生，通过实时聚合酶链反应（PCR）检测细胞中miRNA-146a的表达。
与PBS对照组相比，LPS处理组的TNF-α含量（ng/L）显著增加（650.26±40.53 vs. 6.23±1.76，P<0.01），LPS处理组的miRNA-146a增加了约（5.33±0.81）倍（P<0.01）。
LPS处理的NR8383细胞中miRNA-146a的表达增加，且miRNA-146a可能参与调节NR8383肺泡巨噬细胞的炎症反应。
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