用于心脏干细胞生态位研究的具有类似人心室结构的新型 3D 培养系统。
Novel 3D culture system with similarities to the human heart for studies of the cardiac stem cell niche.
机构信息
Department of Clinical Chemistry & Transfusion Medicine, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden.
出版信息
Regen Med. 2010 Sep;5(5):725-36. doi: 10.2217/rme.10.63.
AIMS
The aim of this study was to develop a 3D culture system with similarities to the human heart, which was suitable for studies of adult cardiac stem or progenitor cells.
MATERIALS & METHODS: Dissociated cells from human cardiac biopsies were placed in high-density pellet cultures and cultured for up to 6 weeks. Gene and protein expressions, analyzed by quantitative real-time PCR and immunohistochemistry, and morphology were studied in early and late pellets.
RESULTS
Cells cultured in the 3D model showed similarities to human cardiac tissue. Moreover, markers for cardiac stem and progenitor cells were also detected after 6 weeks of culture, in addition to markers for signaling pathways active in stem cell niche regulation.
CONCLUSIONS
The described 3D culture model could be a valuable tool when studying the influence of different compounds on proliferation and differentiation processes in cardiac stem or progenitor cells in cardiac regenerative research.
目的
本研究旨在开发一种与人类心脏相似的 3D 培养系统，适用于成人心肌干细胞或祖细胞的研究。
材料与方法
从人类心脏活检中分离出的细胞置于高密度微球培养物中，并培养长达 6 周。通过实时定量 PCR 和免疫组织化学分析来研究早期和晚期微球中的基因和蛋白表达，并研究其形态。
结果
在 3D 模型中培养的细胞与人类心脏组织具有相似性。此外，在培养 6 周后，除了检测到干细胞龛调节中活性的信号通路标志物外，还检测到了心肌干细胞和祖细胞的标志物。
结论
当在心脏再生研究中研究不同化合物对心肌干细胞或祖细胞增殖和分化过程的影响时，所描述的 3D 培养模型可能是一种有价值的工具。
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