Department of Orthopaedic Surgery, Peking University Third Hospital, Beijing, China.
J Surg Oncol. 2010 Dec 1;102(7):838-43. doi: 10.1002/jso.21730.
Programmed Cell Death 5 (PDCD5) is a novel apoptosis-related gene and deregulation of PDCD5 is involved in tumorigenicity. This study was designed to investigate the expression level of PDCD5 and to clarify its clinical significance in chondrosarcoma.
The mRNA and protein levels of PDCD5 in chondrosarcoma and matched corresponding non-tumor tissues were evaluated by real-time PCR and Western blot, respectively. The expression of PDCD5 protein was investigated by immunohistochemistry assays in chondrosarcoma, and its association with clinicopathologic factors and overall survival was also analyzed.
The mRNA and protein levels of PDCD5 were significantly decreased in chondrosarcoma compared with corresponding non-tumor tissues. Low expression of PDCD5 protein was 61.8% (21/34) in chondrosarcomas, as compared 12.5% (1/8) in normal bones, as well as compared 23.5% (4/17) in benign cartilage tumors. PDCD5 expression was correlated with anatomical location and histological grade. The survival rate of patients with low-PDCD5 tumors was lower than that of patients with high-PDCD5 tumors. Multivariate analysis revealed that the downregulated expression of PDCD5 was an independent prognostic factor for overall survival.
PDCD5 is downregulated in chondrosarcoma and might be an independent prognostic factor for overall survival of chondrosarcoma patients.
程序性细胞死亡因子 5（PDCD5）是一种新的凋亡相关基因，其失调与肿瘤发生有关。本研究旨在探讨 PDCD5 在软骨肉瘤中的表达水平及其临床意义。
采用实时 PCR 和 Western blot 分别检测软骨肉瘤及配对非肿瘤组织中 PDCD5 的 mRNA 和蛋白水平。采用免疫组织化学方法检测软骨肉瘤中 PDCD5 蛋白的表达，并分析其与临床病理因素及总生存的关系。
与相应的非肿瘤组织相比，软骨肉瘤中 PDCD5 的 mRNA 和蛋白水平均显著降低。PDCD5 蛋白低表达在软骨肉瘤中的发生率为 61.8%（21/34），而在正常骨中的发生率为 12.5%（1/8），在良性软骨肿瘤中的发生率为 23.5%（4/17）。PDCD5 表达与解剖部位和组织学分级相关。PDCD5 低表达肿瘤患者的生存率低于 PDCD5 高表达肿瘤患者。多因素分析显示，PDCD5 下调是软骨肉瘤患者总生存的独立预后因素。
PDCD5 在软骨肉瘤中下调，可能是软骨肉瘤患者总生存的独立预后因素。
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