Olender Alina, Łetowska Iwona
Katedra i Zakład Mikrobiologii Lekarskiej Uniwersytetu Medycznego w Lublinie.
Med Dosw Mikrobiol. 2010;62(2):135-40.
Wound infections are often due to endogenous bacterial flora which penetrates into a site of injury. The establishment of the etiologic agent can be problematic, especially when opportunistic bacteria are present, suggesting contamination of clinical material. Among bacteria that can cause such diagnostic problems are opportunistic Corynebacterium spp. and coryneforms colonizing skin. The aim of the study was to analyze the 24 clinical samples collected from wounds of different location, with Gram positive rods isolated in numbers suggesting the cause of infection. Bacterial identification was performed by API Coryne and additional biochemical tests (API ZYM, API NE). It was detected that the commonest species isolated were: C. amycolatum (29.2%), C. striatum (16.7%), C. group G (16.7%) and Brevibacterium spp., C. jeikeium, C. urealyticum, C. group F1. The drug susceptibility testing was performed by E-test method. Among isolated strains, 83.3% were simultaneously resistant to erythromycin and clindamycin. In 75% cases resistance to co-trimoxazole was noted, in 71.7% resistance to chloramphenicol and in 16.7% resistance to beta-lactams were detected. In presented study the high percentage of strains resistant to macrolids and linkosamids (MLSB) was noted. All strains were susceptible to vancomycin and teicoplanin.
伤口感染通常是由侵入损伤部位的内源性细菌菌群引起的。病原体的确立可能存在问题,尤其是当存在机会性细菌时,这表明临床材料受到了污染。可能导致此类诊断问题的细菌包括机会性棒状杆菌属和定殖于皮肤的棒状杆菌样细菌。本研究的目的是分析从不同部位伤口采集的24份临床样本,其中分离出的革兰氏阳性杆菌数量提示为感染原因。通过API Coryne和其他生化试验(API ZYM、API NE)进行细菌鉴定。检测到分离出的最常见菌种为:无糖棒状杆菌(29.2%)、纹带棒状杆菌(16.7%)、G群棒状杆菌(16.7%)以及短杆菌属、杰氏棒状杆菌、解脲棒状杆菌、F1群棒状杆菌。采用E-test法进行药敏试验。在分离出的菌株中,83.3%同时对红霉素和克林霉素耐药。75%的病例对复方新诺明耐药,71.7%对氯霉素耐药,16.7%对β-内酰胺类耐药。在本研究中,注意到对大环内酯类和林可酰胺类(MLSB)耐药的菌株比例较高。所有菌株对万古霉素和替考拉宁敏感。