分析印度本土牛品种（Bos indicus）中催乳素-RsaI（PRL-RsaI）基因座的遗传变异。

Analysis of genetic variation at the prolactin-RsaI (PRL-RsaI) locus in Indian native cattle breeds (Bos indicus).

机构信息

Cattle Genomics Lab, National Bureau of Animal Genetics Resources, Karnal, Haryana, India.

出版信息

Biochem Genet. 2011 Feb;49(1-2):39-45. doi: 10.1007/s10528-010-9383-7. Epub 2010 Sep 29.

PMID:20878545

Abstract

This study assessed the distribution pattern of allelic variants at the prolactin-RsaI locus in 23 Indian native cattle breeds (Bos indicus). PCR-RFLP genotyping of a 156 bp fragment of prolactin (PRL) in exon 3 revealed the predominance of the heterozygous AB genotype (mean frequency 0.58) irrespective of utility type (dairy, dual, draft), geographic region (northern, central, southern), and coat color (red, gray) of the breeds analyzed. The overall frequencies of homozygous AA (0.22) and BB (0.20) genotypes were in a similar range. The PRL (A) and PRL (B) alleles exhibited similar gene frequencies (means 0.52 and 0.48, respectively). The existing profile of the PRL-RsaI gene locus in a large set of Indian native cattle breeds was different from that of Bos taurus and cattle breeds of other countries, where either the BB genotype and PRL (B) allele or the AA genotype and PRL (A) allele have been reported to be more prevalent.

摘要

本研究评估了催乳素-RsaI 基因座等位变异在 23 个印度本土牛品种（Bos indicus）中的分布模式。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对第 3 外显子中 156bp 催乳素（PRL）片段进行基因分型，发现无论利用类型（乳用、两用、役用）、地理区域（北部、中部、南部）和品种被毛颜色（红色、灰色）如何，杂合 AB 基因型（平均频率 0.58）均占优势。纯合 AA（0.22）和 BB（0.20）基因型的总体频率处于相似范围。PRL（A）和 PRL（B）等位基因的基因频率相似（均值分别为 0.52 和 0.48）。在一组大型印度本土牛品种中，PRL-RsaI 基因座的现有图谱与Bos taurus 和其他国家的牛品种不同，后者报道 BB 基因型和 PRL（B）等位基因或 AA 基因型和 PRL（A）等位基因更为普遍。