Koziorowska-Gilun M, Koziorowski M, Fraser L, Strzeżek J
Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Reprod Domest Anim. 2011 Jun;46(3):527-33. doi: 10.1111/j.1439-0531.2010.01701.x. Epub 2010 Sep 29.
Antioxidants secreted by the reproductive tract protect spermatozoa against the toxic effects of reactive oxygen species (ROS) after ejaculation. This study aimed at characterizing the level of antioxidant protection in boar cauda epididymidal spermatozoa and fluids of the cauda epididymidis, vesicular and prostate glands. Also, this study investigated the effect of a 5-h period of dialysis on the antioxidant capacity of boar seminal plasma. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) activities were monitored in the cauda epididymidal spermatozoa or reproductive tract fluids. Also, the concentrations of total glutathione (GSH + GSSG), L-ergothioneine (ERT) and l-ascorbate and the total antioxidant status (TAS) of the fluids were measured. It was found that the cauda epididymidal spermatozoa exhibited high SOD activity and relatively low activity of PHGPx. The relative amounts of GPx, GR and GST activities in the cauda epididymidal spermatozoa were negligible, whereas CAT activity was undetectable. Greater SOD activity was found in the fluids of the cauda epididymidis and prostate gland. Furthermore, the prostate gland fluid appeared to be the main source of CAT activity in the seminal plasma, whereas the highest level of GPx activity was derived from the cauda epididymidal fluid. The reproductive tract fluids exhibited negligible amounts of GR and GST activities. It seemed that the significant amounts of GSH + GSSG, ERT and L-ascorbate in the reproductive tract fluids could have an ameliorative effect on the level of TAS in the seminal plasma. Dialysis had a marked effect on the total antioxidant capacity of the seminal plasma, which was manifested in greater activity of SOD and GPx. The findings of this study confirmed that the scavenging potential of the seminal plasma is dependent on the contributions of different antioxidants, originating in various fluids of boar reproductive tract.
生殖道分泌的抗氧化剂可保护精子免受射精后活性氧(ROS)的毒性影响。本研究旨在表征公猪附睾尾部精子以及附睾尾部、精囊和前列腺液中的抗氧化保护水平。此外,本研究还调查了5小时透析期对公猪精浆抗氧化能力的影响。监测了附睾尾部精子或生殖道液中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)、谷胱甘肽转移酶(GST)和磷脂氢过氧化物谷胱甘肽过氧化物酶(PHGPx)活性。此外,还测量了各液体中总谷胱甘肽(GSH + GSSG)、L-麦角硫因(ERT)和L-抗坏血酸的浓度以及液体的总抗氧化状态(TAS)。结果发现,附睾尾部精子表现出较高的SOD活性和相对较低的PHGPx活性。附睾尾部精子中GPx、GR和GST活性的相对量可忽略不计,而CAT活性无法检测到。在附睾尾部和前列腺液中发现了更高的SOD活性。此外,前列腺液似乎是精浆中CAT活性的主要来源,而最高水平的GPx活性则来自附睾尾部液。生殖道液中GR和GST活性的量可忽略不计。似乎生殖道液中大量的GSH + GSSG、ERT和L-抗坏血酸可能对精浆中的TAS水平有改善作用。透析对公猪精浆的总抗氧化能力有显著影响,表现为SOD和GPx活性增强。本研究结果证实,精浆的清除潜力取决于源自公猪生殖道不同液体的不同抗氧化剂的贡献。