Biocenter, Section for Bioinformatics, Innsbruck Medical University, Innsbruck, Austria.
PLoS One. 2010 Sep 24;5(9):e13013. doi: 10.1371/journal.pone.0013013.
Tetracycline regulated ectopic gene expression is a widely used tool to study gene function. However, the tetracycline regulator (tetR) itself has been reported to cause certain phenotypic changes in mammalian cells. We, therefore, asked whether human myeloid U937 cells expressing the tetR in an autoregulated manner would exhibit alterations in gene expression upon removal of tetracycline.
METHODOLOGY/PRINCIPAL FINDINGS: Microarray analyses revealed that 172 and 774 unique genes were significantly differentially expressed by at least 2- or 1.5-fold, respectively, when tetR expressing U937 cells were maintained in media with or without the antibiotic.
CONCLUSIONS/SIGNIFICANCE: These alterations in gene expression are likely to contribute to the phenotypic consequences of tetR expression. In addition, they need to be taken into consideration when using the tetR system for the identification of target genes of transcription factors or other genes of interest.
四环素调控的异位基因表达是研究基因功能的一种广泛应用的工具。然而，四环素调控蛋白（tetR）本身已被报道会导致哺乳动物细胞出现某些表型变化。因此，我们想知道，以自调控方式表达 tetR 的人髓系 U937 细胞在去除四环素后，其基因表达是否会发生改变。
方法/主要发现：微阵列分析显示，当表达 tetR 的 U937 细胞在含有或不含有抗生素的培养基中培养时，分别有 172 个和 774 个独特的基因的表达水平至少相差 2 倍或 1.5 倍。
结论/意义：这些基因表达的改变可能是 tetR 表达的表型后果的原因之一。此外，在使用 tetR 系统来鉴定转录因子的靶基因或其他感兴趣的基因时，需要考虑到这些改变。
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