使用原子力显微镜（AFM）对成纤维细胞进行成像。

Imaging fibroblast cells using atomic force microscopy (AFM).

作者信息

Plodinec Marija, Loparic Marko, Aebi Ueli

出版信息

Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5500. doi: 10.1101/pdb.prot5500.

PMID:20889697

Abstract

Atomic force microscopy (AFM) can be used to visualize the three major cytoskeletal components that contribute to the mechanical properties of the cell. These are actin microfilaments, intermediate filaments, and microtubules. In this protocol, rat embryonic fibroblasts expressing actin tagged with green fluorescent protein (GFP) are used to demonstrate this procedure.

摘要

原子力显微镜（AFM）可用于观察对细胞力学特性有贡献的三种主要细胞骨架成分。它们是肌动蛋白微丝、中间丝和微管。在本实验方案中，使用表达绿色荧光蛋白（GFP）标记的肌动蛋白的大鼠胚胎成纤维细胞来演示此过程。