Faculty of Science, University of Douala, Douala, Cameroon.
Am J Trop Med Hyg. 2010 Oct;83(4):906-8. doi: 10.4269/ajtmh.2010.10-0153.
Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a new light-emitting, diode-based, fluorescence microscope, to compare auramine-rhodamine fluorescence with the conventional Ziehl-Neelsen staining method. Five fluorescence protocols were tested to reduce manipulation time. Smear positivity for acid-fast bacilli with the Ziehl-Neelsen staining method was 27.7% (83 of 300) compared with 33.3% (100 of 300) with the fluorescent method. Staining time with the modified fluorescence protocol could be reduced from 21 minutes to 10 minutes. This study confirmed that the fluorescence staining method is more sensitive than the Ziehl-Neelsen staining method. It is suggested that the training of laboratory technicians on fluorescence microscopy should be scaled up for increased disease control.
由于实验室设备简陋，人力资源匮乏，全球范围内难以大规模开展肺结核（TB）显微镜诊断工作。本研究使用 CyScope®，一种新型基于发光二极管的荧光显微镜，对来自喀麦隆的 300 例痰标本进行研究，将金胺-罗丹明荧光法与传统的齐-尼染色法进行比较。共测试了 5 种荧光方案以减少操作时间。与齐-尼染色法相比，荧光法检测抗酸杆菌的阳性率为 33.3%（300 例中的 100 例），而 Ziehl-Neelsen 染色法为 27.7%（300 例中的 83 例）。改良荧光方案的染色时间可从 21 分钟缩短至 10 分钟。本研究证实荧光染色法比齐-尼染色法更敏感。建议扩大对荧光显微镜技术人员的培训，以加强疾病控制。
