Ismail S M, Gupta A D
School of Life Sciences, University of Hyderabad, India.
Biochem Int. 1990 Oct;22(2):261-8.
Evidence is presented here to show that 20-hydroxyecdysone is essential for the activation of the larval fat body for differential uptake of larval haemolymph proteins (LHPs). By using radiolabelled LHPs it is shown that the fat body cells of Corcyra cephalonica selectively incorporate LHPs during late-larval and prepupal development. Fluorographic analysis of the labelled fat body proteins from prepupal stage separated on sodium dodecyl-sulphate polyacrylamide gels suggests that the LHPs are sequestered without any degradation. Although, during the last larval instar the uptake of all the three LHPs (LHP 1, LHP 2 and LHP 3) by the fat body cells is very low, 20-hydroxyecdysone treatment of early, mid or late-last instars causes a significant increase in uptake of all the three LHPs. However, the response to hormone treatment was more pronounced in late-last instar when compared to early and mid-last instar.
此处提供的证据表明，20-羟基蜕皮酮对于激活幼虫脂肪体以实现对幼虫血淋巴蛋白（LHPs）的差异摄取至关重要。通过使用放射性标记的LHPs，研究表明米蛾的脂肪体细胞在幼虫后期和蛹前期发育过程中选择性地摄取LHPs。对在十二烷基硫酸钠-聚丙烯酰胺凝胶上分离的蛹前期标记脂肪体蛋白进行荧光显影分析表明，LHPs被隔离且未发生任何降解。尽管在最后一龄幼虫期间，脂肪体细胞对所有三种LHPs（LHP 1、LHP 2和LHP 3）的摄取非常低，但对最后一龄幼虫的早期、中期或晚期进行20-羟基蜕皮酮处理会导致所有三种LHPs的摄取显著增加。然而，与最后一龄幼虫的早期和中期相比，在最后一龄幼虫晚期对激素处理的反应更为明显。
