对复制后修复的抑制以及从转化的仓鼠肾细胞中增强SV40病毒的诱导。

Inhibition of postreplication repair and the enhancement of induction of SV40 virus from transformed hamster kidney cells.

作者信息

Zamansky G B, Little J B, Black P H, Kaplan J C

出版信息

Mutat Res. 1978 Jul;51(1):109-19. doi: 10.1016/0027-5107(78)90013-1.

PMID:209322

Abstract

The induction by ultraviolet light of simian virus 40 (SV40) from two SV40--transformed hamster kidney cell lines is enhanced by caffeine. In order to investigate the mechanism responsible for this enhancement, the effect of caffeine on postreplication repair of DNA damaged by UV light was studied utilizing alkaline sucrose-gradient sedimentation. Caffeine at concentrations of 0.5, 1.0 or 2.0 mM inhibited the filling of gaps during postreplication repair. In addition, caffeine was found to potentiate cell killing by mitomycin C, an alkylating agent, and to enhance SV40 induction by mitomycin C. We postulate that the persistence of gaps in DNA, caused by the presence of caffeine, results in the enhancement of SV40 virus induction.

摘要

咖啡因可增强紫外线对两种经猿猴病毒40（SV40）转化的仓鼠肾细胞系中SV40的诱导作用。为了探究这种增强作用的机制，利用碱性蔗糖梯度沉降法研究了咖啡因对紫外线损伤DNA复制后修复的影响。浓度为0.5、1.0或2.0 mM的咖啡因会抑制复制后修复过程中缺口的填补。此外，还发现咖啡因可增强丝裂霉素C（一种烷化剂）对细胞的杀伤作用，并增强丝裂霉素C对SV40的诱导作用。我们推测，咖啡因的存在导致DNA中缺口持续存在，从而增强了SV40病毒的诱导。