基于菌株 ATCC 8142 的荚膜基因座的 e 型流感嗜血杆菌新型诊断 PCR。

New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142.

机构信息

Institute for Hygiene and Microbiology, Consultant Laboratory for Haemophilus influenzae, University of Würzburg, Josef-Schneider-Str. 2 (E1), D-97080 Würzburg, Germany.

出版信息

Int J Med Microbiol. 2011 Feb;301(2):176-9. doi: 10.1016/j.ijmm.2010.07.004.

DOI:10.1016/j.ijmm.2010.07.004

PMID:20940106

Abstract

A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was >99% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.

摘要

我们开发了一种新的 PCR 方案，用于对 e 型流感嗜血杆菌（Hie）进行分子分型。为此，我们确定了 Hie 菌株 ATCC8142 荚膜区 II 的序列，该序列与最近发表的 Hie 分离株 274 的序列 >99%相同。使用引物对 TTL63/TTL64 的 PCR 可扩增 ecs4 的内部 592bp 片段，ecs4 是一种 e 型特异性荚膜合成基因，在 40 株 Hie 菌株中均有扩增。在所有非 Hie 菌株中，均未出现假阳性。Falla 等人（1994）提出的 PCR 假阴性结果可归因于引物序列中的单个核苷酸插入。