Lee S H, We J S, Sohn W M, Hong S T, Chai J Y
Department of Parasitology, Seoul National University College of Medicine, Korea.
Kisaengchunghak Chapchi. 1990 Sep;28(3):161-73. doi: 10.3347/kjp.1990.28.3.161.
The complete life cycle of Spirometra erinacei has been experimentally maintained in the laboratory. The cyclops were reared as the first intermediate host, and the tadpoles of Rana nigromaculata as the second intermediate host. ICR mice were used as another second host. The experimental definitive hosts were dogs and cats. Maturation and hatching of the eggs took 8 to 14 days by incubation at 29 degrees C. The coracidium measured 43.8 x 36.9 microns. Mesocyclops leuckarti and Eucyclops serrulatus were susceptible to the coracidial infection. The procercoids older than 5 days in the cyclops had minute spines at the anterior end, calcium corpuscles in the body parenchyme and the cercomer at the posterior end. Procercoids 10 to 20 days old were infective to tadpoles, and 15 or 21 day old worms could infect the mice. The plerocercoids from the tadpoles at 15 days after experimental infection were pear-shaped and shorter than 1 mm in the length and were infective to mice. Fifteen to 18 days after experimental inoculation of plerocercoids to dogs or cats, the adult worms began to produce eggs. One life cycle from egg to egg needed 48 to 67 days in the laboratory. The morphology of larval or adult worms was compatible with the description of Spirometra erinacei.
曼氏迭宫绦虫的完整生命周期已在实验室中通过实验维持。剑水蚤作为第一中间宿主进行饲养，黑斑蛙蝌蚪作为第二中间宿主。ICR小鼠用作另一第二宿主。实验终末宿主为狗和猫。卵在29摄氏度下孵育8至14天可成熟并孵化。钩球蚴大小为43.8×36.9微米。吕氏中剑水蚤和锯缘真剑水蚤易受钩球蚴感染。剑水蚤体内5日龄以上的原尾蚴前端有微小的棘，体实质中有钙颗粒，后端有尾球。10至20日龄的原尾蚴可感染蝌蚪，15或21日龄的虫体可感染小鼠。实验感染后15天蝌蚪体内的裂头蚴呈梨形，长度小于1毫米，可感染小鼠。将裂头蚴接种到狗或猫体内15至18天后，成虫开始产卵。在实验室中，从卵到卵的一个生命周期需要48至67天。幼虫或成虫的形态与曼氏迭宫绦虫的描述相符。
