Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Nucl Med. 2010 Nov;51(11):1659-62. doi: 10.2967/jnumed.110.078584. Epub 2010 Oct 18.
Induction of apoptosis is the primary mechanism through which most chemotherapies cause tumor cell death. Early assessment of tumor response is required to manage patients in terms of quality of life versus intensive chemotherapy. Although imaging with radiolabeled annexin V has been intensively investigated, it is still not sufficiently mature for clinical application. This article will summarize various alternative imaging techniques for visualization of phosphatidylserine externalization, activity of caspases, and mitochondrial membrane potential. Such imaging studies will promote the identification of novel molecular targets and the development of highly specific apoptosis-detecting imaging probes with potential clinical applications. It is highly possible that quantitative imaging of apoptosis will greatly improve clinical decision making in apoptosis-related diseases.
诱导细胞凋亡是大多数化疗药物引起肿瘤细胞死亡的主要机制。为了提高患者的生活质量并避免过度化疗，需要早期评估肿瘤的反应。尽管放射性标记的膜联蛋白 V 的成像已得到深入研究，但它仍不够成熟，无法应用于临床。本文将总结各种替代成像技术，用于可视化磷脂酰丝氨酸外翻、半胱天冬酶的活性和线粒体膜电位。此类成像研究将促进新型分子靶标的鉴定以及高特异性凋亡检测成像探针的开发，并具有潜在的临床应用前景。定量成像凋亡极有可能极大地改善与凋亡相关疾病的临床决策。
