Shypshyna M S, Veselovs'kyĭ M S
Fiziol Zh (1994). 2010;56(4):26-36.
We examined properties of chemical neurotransmission at the level of primary afferent inputs into spinal cord with the new easy-to-use in vitro model of contiguously-cultivated dissociated both the dorsal root ganglion neurons (DRG) and the dorsal horn spinal cord neurons (DHSC) from newborn rats. The results of our studies showed the presence of excitatory and inhibitory DRG neurons synapses on the cells of DHSC. The excitatory afferent signaling in such synapses was mediated by presynaptic release of glutamate and a following activation of both NMDA- and non-NMDA-receptor subclasses. In these cases the activation of non-NMDA-receptors makes a main contribution to realization of excitatory postsynaptic effects. Either glycine- or GABA-ergic DRG neurons were involved in transmission of inhibitory signals to the DHSC neurons. However, in vast majority of examined neuronal pairs the inhibitory synaptic transmission was mediated by presynaptic release of glycine. As distinct from the previous similar methods, an in vitro model of co-culture of both the DRG and the DHSC neurons proposed here allows to use comprehensively modem technical approaches for examination of the transmission of somatosensory information from the periphery to the CNS. The described model could be acceptable for detailed investigation of specific properties of primary afferent synapses.
我们使用一种新的、易于使用的体外模型，该模型将新生大鼠的背根神经节神经元（DRG）和脊髓背角神经元（DHSC）连续培养并解离，研究了脊髓初级传入输入水平上的化学神经传递特性。我们的研究结果表明，在DHSC细胞上存在兴奋性和抑制性DRG神经元突触。此类突触中的兴奋性传入信号由谷氨酸的突触前释放以及随后NMDA和非NMDA受体亚类的激活介导。在这些情况下，非NMDA受体的激活对兴奋性突触后效应的实现起主要作用。甘氨酸能或GABA能DRG神经元参与向DHSC神经元传递抑制性信号。然而，在绝大多数检测的神经元对中，抑制性突触传递由甘氨酸的突触前释放介导。与先前类似方法不同，这里提出的DRG和DHSC神经元共培养体外模型允许全面使用现代技术方法来研究躯体感觉信息从外周向中枢神经系统的传递。所描述的模型对于详细研究初级传入突触的特定特性可能是可接受的。
