细胞外环境因素对日本鳗鲡精子运动性的影响。

Effects of extracellular environment factors on the motility in Japanese eel spermatozoa.

机构信息

East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China.

出版信息

Cell Biol Int. 2011 May;35(5):505-8. doi: 10.1042/CBI20100474.

DOI:10.1042/CBI20100474

PMID:20979577

Abstract

We have examined the effects in vitro of the ionic composition, pH and temperature on the motility by the spermatozoa of the Japanese eel (Anguilla japonica). Milt was obtained from 10 males that had been artificially matured by repeated injections of hCG. Sperm motility was monitored with a VHS video recorder and a video camera connected to a microscope. The results showed that most of the sperm were highly motile in 250-700 mM NaCl, 250-650 mM KCl and 350-550 mM CaCl2 solution. The longest duration of sperm motility recorded in 500 mM NaCl, 250 mM KCl and 350 mM CaCl2 solution. Sperm was not motile when suspended at pH 2, sperm motility was observed at pH 3, there was a relatively higher percentage of motile sperm in solutions at pH 4-12 (above 80%). The motility and duration increased within 18-24°C and decreased at the range of 24-30°C. Appropriate K+ ion concentration in the active medium could enhance the percent motility and duration of eel spermatozoa.

摘要

我们研究了离子组成、pH 值和温度对日本鳗鲡（Anguilla japonica）精子体外运动的影响。精液取自 10 条经 hCG 重复注射人工成熟的雄性鳗鱼。精子运动通过 VHS 录像机和显微镜连接的摄像机进行监测。结果表明，在 250-700 mM NaCl、250-650 mM KCl 和 350-550 mM CaCl2 溶液中，大多数精子具有高度运动性。在 500 mM NaCl、250 mM KCl 和 350 mM CaCl2 溶液中记录到的精子运动持续时间最长。当悬浮在 pH 值为 2 时，精子没有运动，在 pH 值为 3 时观察到精子运动，在 pH 值为 4-12（高于 80%）的溶液中存在相对较高百分比的运动精子。运动和持续时间在 18-24°C 范围内增加，在 24-30°C 范围内减少。在活性介质中适当的 K+离子浓度可以提高鳗鱼精子的运动百分比和持续时间。