Miyasaki K T, Bodeau A L, Selsted M E, Ganz T, Lehrer R I
UCLA School of Dentistry, Center for the Health Sciences, Los Angeles.
Oral Microbiol Immunol. 1990 Dec;5(6):315-9. doi: 10.1111/j.1399-302x.1990.tb00432.x.
Oral, gram-negative, facultative bacteria, including Actinobacillus actinomycetemcomitans, Eikenella corrodens, and Capnocytophaga spp. have been associated with destructive periodontal infection. Neutrophils play a critical role in defending the periodontium against destructive infection. Defensins are antimicrobial peptides that have been isolated in human, rabbit, guinea pig, and rat leukocytes that may constitute an important nonoxidative mechanism of killing. The purpose of this study was to examine the sensitivity of a battery of oral, gram-negative, facultative bacteria to the bactericidal effects of the isolated rabbit peptide NP-1. All species tested were killed by NP-1; however, there was strain-to-strain variation in sensitivity. The bactericidal effect was not dependent on net bacterial growth, although metabolic activity was evident as assessed by bacterial oxygen consumption. We conclude that bacteria are sensitive to the cidal mechanism involved in defensin-mediated bacterial killing and that the conditions of this assay system support the killing of bacteria by the defensin peptides.
口腔革兰氏阴性兼性菌，包括伴放线放线杆菌、腐蚀艾肯菌和二氧化碳嗜纤维菌属，与破坏性牙周感染有关。中性粒细胞在保护牙周组织免受破坏性感染方面起着关键作用。防御素是一种抗菌肽，已在人、兔、豚鼠和大鼠白细胞中分离出来，可能构成一种重要的非氧化杀伤机制。本研究的目的是检测一系列口腔革兰氏阴性兼性菌对分离出的兔肽NP-1杀菌作用的敏感性。所有测试菌株均被NP-1杀死；然而，敏感性存在菌株间差异。尽管通过细菌耗氧量评估代谢活性明显，但杀菌作用并不依赖于细菌的净生长。我们得出结论，细菌对防御素介导的细菌杀伤所涉及的杀伤机制敏感，并且该检测系统的条件支持防御素肽对细菌的杀伤作用。
