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[绵羊产后子宫、卵巢的生物测量参数及17β-雌二醇(E2)水平]

[Biometric parameters of the uterus, ovaries and levels of 17 beta-estradiol (E2) after delivery in sheep].

作者信息

Krajnicáková M, Elecko J, Bekeová E, Maracek I, Hendrichovský V

机构信息

Ustav experimentálnej veterinárnej medicíny, Kosice.

出版信息

Vet Med (Praha). 1990 Dec;35(12):747-56.

PMID:2100427
Abstract

Biometric changes of uterus, ovaries, follicles and 17 beta-oestradiol (E2) concentrations were investigated in 15 lambing ewes of the Slovak Merino breed in the puerperal period. The sex organs were excised immediately after bleeding from ewes slaughtered on days 1, 7, 17, 25 and 34 post partum (p. p.). Biometric parameters of the body and horns of uterus were measured by a calliper. The ovaries were weighed on an analytical balance, their length, width and height were measured at the same time. The size and number of follicles were determined on the ovary surface. The blood for E2 detection was collected from vena jugularis three and one day before delivery (days -1, -3). Blood samples were also collected after delivery on days 1, 7, 17, 25 and 34. E2 concentrations in the blood serum of ewes were determined by RIA-test-ESTRA kits, designed in one institute at Kosice. The highest weight of uterus body in the test ewes was recorded on day 1 p. p. In the following days the weight of uterus body had a decreasing trend. There were significant differences in the weight of uterus body from day 17 to day 34 p. p., in comparison with the first day after lambing (P less than 0.01). A significant decrease in the length of uterus body was observed from day 17 to day 34 of observation (P less than 0.01; P less than 0.001). An increase in the length of a nongravid horn, observed on day 7 p. p., was followed by a gradual decrease until day 34, similarly like in its weight. No statistically significant differences were found out in the ovary length, width and height. Neither were any greater changes recorded in the weight of ovaries from day 1 to day 34 after delivery. The highest number of small structures (28) observed on day 7 p. p. in the ipsilateral ovary was decreasing in the course of puerperium and the number of follicles larger than 2, 4 and 5 mm was increasing. The highest concentrations of E2 were not recorded on day -1 before delivery. The significantly lowest concentrations of E2 were recorded on day 25 p. p. The above-mentioned results are preliminary and they enlarge the knowledge of biometric parameters of uterus, ovaries, follicles and E2 concentrations after delivery in ewes.

摘要

对15只斯洛伐克美利奴品种的产羔母羊在产后时期的子宫、卵巢、卵泡和17β-雌二醇(E2)浓度的生物统计学变化进行了研究。在产后第1、7、17、25和34天(p.p.)宰杀母羊放血后立即切除生殖器官。用卡尺测量子宫体和子宫角的生物统计学参数。在分析天平上称量卵巢重量,同时测量其长度、宽度和高度。在卵巢表面确定卵泡的大小和数量。在分娩前3天和1天(-1、-3天)从颈静脉采集用于检测E2的血液。在产后第1、7、17、25和34天也采集血样。母羊血清中的E2浓度通过科希策一所研究所设计的RIA-test-ESTRA试剂盒测定。试验母羊子宫体的最高重量记录在产后第1天。在接下来的日子里,子宫体的重量呈下降趋势。与产羔后第一天相比,产后第17天至第34天子宫体的重量有显著差异(P<0.01)。在观察的第17天至第34天,观察到子宫体长度显著下降(P<0.01;P<0.001)。产后第7天观察到非妊娠子宫角长度增加,随后逐渐下降直至第34天,其重量变化情况类似。在卵巢长度、宽度和高度方面未发现统计学上的显著差异。产后第1天至第34天卵巢重量也未记录到更大变化。产后第7天在同侧卵巢观察到的小结构数量最多(28个),在产褥期过程中逐渐减少,而直径大于2、4和5毫米的卵泡数量在增加。分娩前-1天未记录到E2的最高浓度。产后第25天记录到E2的显著最低浓度。上述结果为初步结果,它们扩展了对母羊产后子宫、卵巢、卵泡的生物统计学参数和E2浓度的认识。

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