Giryn H, Gruszczyńska Z
Zakładu Analizy Zywności Instytutu Biotechnologii Przemysłu Rolno-Spozywczego.
Rocz Panstw Zakl Hig. 1990;41(5-6):217-22.
Conditions of simultaneous determination of benzoic and sorbic acid in orange drink and tomato concentrate by gas chromatography method were developed and tested. Preservatives were isolated by extraction of the samples with diethyl ether in acidic media, and then converted into methyl esters with thionyl chloride. Methyl esters were separated on column packed with 15% EGA coated on 80-100 mesh Chromosorb W AW DMCS. The smallest amount of preservatives detectable on chromatography column is 20 ng for benzoic acid and 16 ng for sorbic acid. The recovery rate of the method ranged from 96-97% for benzoic acid and 89-92% for sorbic acid, while the variability coefficient was from 1.7-3.5% for benzoic acid and 4.5-6% for sorbic acid.
建立并测试了用气相色谱法同时测定橙汁饮料和浓缩番茄汁中苯甲酸和山梨酸的条件。在酸性介质中用乙醚萃取样品分离出防腐剂，然后用亚硫酰氯将其转化为甲酯。甲酯在填充有涂覆在80 - 100目Chromosorb W AW DMCS上的15% EGA的色谱柱上进行分离。色谱柱上可检测到的防腐剂最小量，苯甲酸为20 ng，山梨酸为16 ng。该方法的回收率，苯甲酸为96 - 97%，山梨酸为89 - 92%，而变异系数，苯甲酸为1.7 - 3.5%，山梨酸为4.5 - 6%。
