Pogorelov A G, Pogorelova V N, Pogorelova M A
Biofizika. 2010 Sep-Oct;55(5):875-9.
Electron probe microanalysis was applied to determine cytoplasmic elemental (K, Na, Cl) concentrations in cardiac cells of the rat (Wistar). Potassium, sodium and chlorine contents were measured in papillary muscle myocytes of the rat heart perfused by the Langendorff's procedure. Ischemic depletion was created by perfusion with deeply deoxygenated Tirode's solution in the absence of glucose. It was found that the initial phase of acute ischemia is characterized by the potassium deficiency and the accumulation of sodium and chlorine in cardiac myocytes. It should be noted that changes in the total charge of the main intracellular cations (K+, Na+) do not compensate for the increased chlorine concentration. This result can be accounted for by the appearance of ionic (K+ and Cl-) transport coupled with the removal of lactate anions produced in cardiomyocytes during anaerobic glycolysis.
应用电子探针微分析技术测定大鼠（Wistar）心脏细胞胞质中的元素（钾、钠、氯）浓度。采用Langendorff灌流法对大鼠心脏乳头肌心肌细胞中的钾、钠和氯含量进行测定。在无葡萄糖的情况下，用深度脱氧的台氏液灌流造成缺血性耗竭。结果发现，急性缺血的初始阶段表现为心肌细胞中钾缺乏以及钠和氯的蓄积。应当指出，主要细胞内阳离子（K+、Na+）总电荷的变化并不能补偿氯浓度的升高。这一结果可通过离子（K+和Cl-）转运的出现来解释，该转运与无氧糖酵解过程中心肌细胞产生的乳酸阴离子的清除相关。
