State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
Bioresour Technol. 2011 Jan;102(2):1815-21. doi: 10.1016/j.biortech.2010.09.048. Epub 2010 Sep 17.
To improve 1,3-propanediol (1,3-PD) production by an economic and efficient approach, hemicellulosic hydrolysates (HH) used as cosubstrate resulted in more biomass and higher reducing power for 1,3-PD production. The effects of primary degradation products such as individual sugars (xylose, glucose, mannose, arabinose and galactose) and major inhibitors (furfural, acetate and formate) on the Klebsiella pneumoiae growth and 1,3-PD production were investigated in this study. Xylose and mannose could efficiently promote the 1,3-PD production and cell growth. Furfural (0.28 g/l) and sodium acetate (1.46 g/l) in low concentration were not inhibitory to Klebsiella pneumoniae, rather they have stimulatory effect on the growth and 1,3-PD biosynthesis, especially the acetate. In fed-batch fermentation with HH as cosubstrate, the final 1,3-PD production, conversion from glycerol and productivity were 71.58 g/l, 0.65 mol/mol and 1.93 g/l/h, respectively, which were 17.8%, 25.0% and 17.7% higher than that from glycerol alone.
为了通过经济高效的方法提高 1,3-丙二醇（1,3-PD）的产量，将半纤维素水解物（HH）用作共底物，这导致了更多的生物量和更高的还原力用于 1,3-PD 的生产。本研究考察了主要降解产物（如木糖、葡萄糖、甘露糖、阿拉伯糖和半乳糖）和主要抑制剂（糠醛、乙酸盐和甲酸盐）对肺炎克雷伯氏菌生长和 1,3-PD 生产的影响。木糖和甘露糖可以有效地促进 1,3-PD 的生产和细胞生长。低浓度的糠醛（0.28 g/l）和乙酸钠（1.46 g/l）对肺炎克雷伯氏菌没有抑制作用，反而对生长和 1,3-PD 生物合成有刺激作用，特别是乙酸盐。在以 HH 为共底物的分批补料发酵中，最终 1,3-PD 产量、甘油转化率和生产率分别为 71.58 g/l、0.65 mol/mol 和 1.93 g/l/h，比单独使用甘油时分别提高了 17.8%、25.0%和 17.7%。
